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HDAC Assay Buffer

EAA 10 X
Catalog No. ABIN925590
  • Application
    Enzyme Activity Assay (EAA)
    Characteristics
    Sterilization: Sterile Aseptic Filter
    HDAC Assay Buffer is typically employed with appropriate standards and controls.
    Synonyms: HDAC Activity Assay Kit
  • Application Notes
    This product is a 10X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl and 1.0 mM MgCl2 at pH 8.0.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 X
    Storage
    -20 °C
  • Background
    Histone deacetylases (HDACs) are typically found in multiprotein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. HDAC assays allow the determination of deacetylase activity and provide accurate activity information that is reflective of endogenous regulation.
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