PEB (4x), protein extraction buffer

Catalog No. ABIN618925

Product Details

Application: Protein Extraction (PEx)

Characteristics: PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure) depending on the starting material.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4 X

Buffer: Buffer containing approx. 40 % v/v glycerol, TrisHCl pH 8.5, LDS, EDTA

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

Storage: 4 °C