FACS and IF Staining Buffer with Goat Serum

Catalog No. ABIN5706393

Product Details

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC)

Purification: FACS and IF Staining Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Aseptic filtered

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is adequate for cell staining, washing and dilution in flow cytometry and immunofluorescence experiments.

IF Microscopy Dilution: User Optimized

Comment:

Synonyms: Fluorescent Activated Cell Sorting (FACS), Staining buffer, Flow cytometry staining buffer, Immunofluorescence staining buffer

Background: Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is a diluent buffer optimized for use in flow cytometry and immunofluorescence assay of viable and/or fixed cells. FACS and IF staining, washing buffer contains 2 % goat serum as carrier and stabilizer to reduce non-specific binding of antibodies and fluorochrome reagents to targets cells. Metabolic inhibitors and NaN3 are also included in the flow cytometry and immunofluorescence buffer to prevent patching, capping of surface antigen and bacterial growth.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 X

Buffer:

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 3 months