Express Transfer Buffer

Catalog No. ABIN5706384

Product Details

Application: Western Blotting (WB)

Purification: This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Sterility: Aseptic filtered

Application Details

Application Notes:

Application Note: Express Transfer Buffer is a alternative to tris-glycine as a transfer buffer in the western blot. It may run at an elevated temperature but gives markedly lower background.

Western Blot Dilution: User Optimized

Comment:

Synonyms: Carbonate Buffer, Western Blot Buffer, non-tris-glycine buffer

Background: Western Blotting is the method used for immunodetection for the detection of proteins. Blotting is a method for transferring biological molecules (e.g., proteins, nucleic acid fragments) from a gel matrix to a membrane support for the subsequent detection of those molecules.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 X

Buffer:

Stabilizer: None

Preservative: Without preservative

Storage: RT,4 °C

Expiry Date: 3 months