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TMB Membrane Peroxidase Substrate (Ready-To-Use)

DB, WB 1 X
Catalog No. ABIN925633
  • Application
    Dot Blot (DB), Western Blotting (WB)
    Characteristics
    TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is a chromogenic substrate used to visualize antibody reactivity in ELISA experiments. In the presence of peroxidases, TMB can act as an electron donor for the conversion of peroxides to water, changing the color of solution to a blue color equivalent to the degree of reactivity. This reaction of TMB can be halted with acid to change the TMB to yellow. TMB Membrane Peroxidase Substrate (3, 3', 5, 5' - Tetramethylbenzidine) is ideal for investigators in Immunology, Cancer, and Microbiology research.
    Synonyms: 3,3’,5,5’-Tetramethylbenzidine, TMB-M, TMBM
  • Application Notes
    TMB Membrane Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB Membrane Peroxidase Substrate comes ready to use.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 X
    Storage
    4 °C
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