Loading Buffer (5X)
-
- Characteristics
- Loading buffer for separation and visualization proteins with SDS-PAGE and Western Blot analysis.
-
-
- Application Notes
- Sample can be prepared under reducing conditions with the addition of a reducing agent such as beta-mercaptoethanol or DTT. Dilute to 1X in sample. Do not heat prior to loading on gel. For reducing buffer, add 5 % (v/v) beta-mercaptoethanol or 1 mM DTT to 1X loading buffer and sample. Boil sample/loading buffer for 5 min. Do not store buffer with reducing agent, add fresh prior to each use.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Storage
- -20 °C
- Storage Comment
-
Short Term Storage: +20°C
Long Term Storage: +20°C
Stable for at least 2 years after receipt when stored at +20°C. - Expiry Date
- 24 months
-