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Loading Buffer (5X)

Catalog No. ABIN5564173
  • Characteristics
    Loading buffer for separation and visualization proteins with SDS-PAGE and Western Blot analysis.
  • Application Notes
    Sample can be prepared under reducing conditions with the addition of a reducing agent such as beta-mercaptoethanol or DTT. Dilute to 1X in sample. Do not heat prior to loading on gel. For reducing buffer, add 5 % (v/v) beta-mercaptoethanol or 1 mM DTT to 1X loading buffer and sample. Boil sample/loading buffer for 5 min. Do not store buffer with reducing agent, add fresh prior to each use.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    -20 °C
    Storage Comment
    Short Term Storage: +20°C
    Long Term Storage: +20°C
    Stable for at least 2 years after receipt when stored at +20°C.
    Expiry Date
    24 months
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