RBC Lysis/Fixation Solution (10X)
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- Application
- Intracellular Flow Cytometry (ICFC)
- Characteristics
- RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H2 0 prior to use.
- Components
- RBC Lysis/Fixation Solution (10X)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 10 X
- Buffer
- This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water. The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
- Storage
- 4 °C
- Storage Comment
- Store RBC lysis buffer between 2°C and 8°C.
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