ELISA Assay Diluent (5X)
MA, ELISA
5 X
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- Application
- Multiplex Assay (MA), ELISA
- Characteristics
- ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.
- Components
- ELISA Assay Diluent (5X)
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
RIPA Lysis BufferIP, WB 10 X
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- Application Notes
- Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 5 X
- Storage
- 4 °C
- Storage Comment
- This solution can be stored unopened between 2°C and 8°C. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage. Warm ELISA reagents to room temperature (18-25°C) before use.
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