Fixation Buffer

Catalog No. ABIN1379871

Product Details

Application: Intracellular Flow Cytometry (ICFC)

Brand: BD Cytofix™

Characteristics: BD Cytofix™ Fixation Buffer is comprised of a neutral pH-buffered saline (i.e., Dulbecco's Phosphate-Buffered Saline) that contains 4% w/v paraformaldehyde. This fixation buffer is intended to preserve human and rodent lymphoid cells for the subsequent immunofluorescent staining of intracellular cytokines. BD Cytofix can also be used to preserve the light-scattering characteristics and fluorescence intensities of human and rodent hematopoietic cells that have been stained by immunofluorescence for subsequent flow cytometric analysis.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).

Lanier, Warner: "Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis." in: Journal of immunological methods, Vol. 47, Issue 1, pp. 25-30, (1982) (PubMed).