Soluble Protein Master Buffer Kit

Catalog No. ABIN1379784

Product Details

Reactivity: Mouse, Rat

Application: Immunoassay (IA), Flow Cytometry (FACS)

Brand: BD CBA™

Characteristics: The BD™ CBA Mouse or Rat Soluble Protein Flex Set and BD™ CBA Mouse/Rat Soluble Protein Master Buffer Kit employ particles with discrete fluorescence intensities to detect soluble analytes at very low concentrations. The working assay range for most analytes in this system is 10 to 2,500 pg/mL for mouse assays and 40 to 10,000 pg/mL for rat assays.
The BD CBA Mouse/Rat Soluble Protein Master Buffer Kit contains all of the supporting reagents necessary to perform an assay using a BD CBA Mouse or Rat Soluble Protein Flex Set. The buffers and instrument setup reagents provided in this kit have been optimized for analysis of analytes in tissue culture supernatants, plasma, and serum samples.

Components: Assay diluent, capture bead diluent, capture bead diluent for serum/plasma samples, detection reagent diluent, wash buffer, instrument setup reagents

Application Details

Comment:

Required for use with BD™ CBA Mouse/Rat Soluble Protein Flex Sets

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References

Cook, Stahl, Lowe, Chen, Morgan, Wilson, Varro, Chan, Graziano, Barney: "Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics." in: Journal of immunological methods, Vol. 254, Issue 1-2, pp. 109-18, (2001) (PubMed).

Dotti, Savoldo, Takahashi, Goltsova, Brown, Rill, Rooney, Brenner: "Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells. A model for immunotherapy." in: Experimental hematology, Vol. 29, Issue 8, pp. 952-61, (2001) (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

Carson, Vignali: "Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 41-52, (1999) (PubMed).

Chen, Lowe, Wilson, Crowther, Tzeggai, Bishop, Varro: "Simultaneous Quantification of Six Human Cytokines in a Single Sample Using Microparticle-based Flow Cytometric Technology." in: Clinical chemistry, Vol. 45, Issue 9, pp. 1693-1694, (1999) (PubMed).

Bishop, Davis: "A flow cytometric immunoassay for beta2-microglobulin in whole blood." in: Journal of immunological methods, Vol. 210, Issue 1, pp. 79-87, (1998) (PubMed).

Oliver, Kettman, Fulton: "Multiplexed analysis of human cytokines by use of the FlowMetrix system." in: Clinical chemistry, Vol. 44, Issue 9, pp. 2057-60, (1998) (PubMed).