CHAPS Buffer
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- Application
- Immunoprecipitation (IP)
- Characteristics
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Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis.
Effective as a stand alone reagent for cell lysis and protein extraction.
Contains a non-amine buffer enabling cross-linking with NHS-ester derivatives.
Minimal interference with Bradford and Lowry protein assays.
60 mL volume: Suitable for two hundred 10-cm culture plates, or 600 wells in 6-well dishes.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
RIPA Lysis BufferIP, WB 10 X
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- Comment
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Properties of CHAPS Lysis and Immunoprecipitation BufferCHAPS is a zwitterionic detergent with strong membrane dissolution capacity while maintaining protein conformation and protein-protein interaction. CHAPS reagent provides an effective stand alone buffer for cell lysis and protein extraction as well as a reagent suitable for downstream immunoprecipitation-pull down approaches. The standard FIVEphoton Biochemicals CHAPS reagent is provided without calcium chloride or EDTA.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 %
- Buffer
- CHAPS based cell lysis and immunoprecipitation buffer containing 0.5% CHAPS, pH 8.0.
- Storage
- 4 °C
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