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- Target
- Biotin Ligase Tag (BLT) (BLT) (Biotin Ligase Tag (BLT))
- Host
- Rabbit
- Clonality
- Polyclonal
- Sequence
- GLNDIFEAQK IEWH
- Specificity
- This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA. This peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli. Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).
- Characteristics
- N/A
- Immunogen
-
GLNDIFEAQKIEWH (minimal BirA substrate, biotin is added to the sequence at the lysine.)
Immunogen Type: Peptide
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- Comment
-
Gene Name: BirA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 90 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
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- Target
- Biotin Ligase Tag (BLT) (BLT) (Biotin Ligase Tag (BLT))
- Target Type
- Tag
- Background
-
N/A
Synonyms: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-[acetyl-CoA carboxylase] synthetase.
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