Catcher-Control
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- Application
- Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Protein Complex Immunoprecipitation (Co-IP)
- Purpose
- Catcher-Control is a control resin for all Catcher resins, which are based on 4 % crosslinked agarose beads.
- Characteristics
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Catcher-Control is a control resin for all Catcher resins, which are based on 4 % cross-linked agarose beads. It was prepared using the same activation and quenching chemistry that was used for preparation of Catcher resins, but is not charged with any single-domain antibody.
Catcher-Control is thus the ideal resin for pre-depletion of extracts or as a side-by-side specificity control for any Catcher resins.
When using as a specificity control, Catcher-Control and the corresponding sdAb-charged Catcher Resin should be used under identical experimental conditions. - Bead Matrix
- Agarose beads
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Magnetic Concanavalin A Beads (Agarose)
CUT&RUN, CUT&Tag, Sep Concanavalin A Magnetic Agarose beads 30 μm
Ni-NTA MagBeadsPull-Down, Purif, Sep Ni-NTA 25 µm
Ni-NTA AgarosePurif, Sep Ni-NTA 40 µm
Rho1D4 AgarosePurif, Sep Rho1D4 40 µm
BFP-CatcherRIP, Co-IP, IP, Purif, ChIP Antibody Agarose beads 90 μm
INDIGO Ni-AgarosePurif Ni-INDIGO Agarose beads 100 μm
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- Application Notes
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Coating: Same chemistry as for Catcher resins, but without immobilized single-domain antibody.
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Buffer Compatibility:- Common buffer substances at pH 5 to 9
- 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
- 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
- 4 M urea
- 10 mM DTT, 10 mM 2-Mercaptoethanol
- RNAse A, DNAse I, Benzonase, protease inhibitors
- Restrictions
- For Research Use only
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- Buffer
- 50 % slurry in PBS containing 20 % Ethanol
- Storage
- 4 °C
- Storage Comment
- Store at 4 °C, do not freeze
- Expiry Date
- 12 months
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