Protein A/G Magnetic Beads

Catalog No. ABIN400577

Product Details

Target: Protein A/G

Application: Immunoprecipitation (IP), Purification (Purif), Affinity Chromatography (AC)

Purpose: Protein A/G MagBeads are designed for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens.

Brand: MagBeads

Characteristics: Protein A/G MagBeads are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein A/G. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).

Bead Ligand: Protein A,Protein G

Bead Matrix: Magnetic particles

Bead Size: 40 µm

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.5 ml settled Beads (2 ml 25% slurry)

Storage: 4 °C

Storage Comment: Store at 4°C, do NOT freeze.

Expiry Date: 12 months

References

Tyler, Pearce, Shaler, Olzmann, Greenblatt, Kopito: "Unassembled CD147 is an endogenous endoplasmic reticulum-associated degradation substrate." in: Molecular biology of the cell, Vol. 23, Issue 24, pp. 4668-78, (2012) (PubMed).

Luo, Hannemann, Kulkarni, Schwartz, ODowd, Fortunato: "Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells." in: Journal of virology, Vol. 84, Issue 7, pp. 3528-41, (2010) (PubMed).

Target Details

Target: Protein A/G

Abstract: Protein A/G Products

Background: Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.