Protein A Magnetic Beads

Catalog No. ABIN400575

Product Details

Target: Protein A

Reactivity: Staphylococcus aureus

Application: Immunoprecipitation (IP), Purification (Purif), Affinity Chromatography (AC)

Purpose: Protein A MagBeads are designed for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens.

Brand: MagBeads

Characteristics: Protein A MagBeads are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein A. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A MagBeads have a binding capacity of more than 10 mg Rabbit IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).

Bead Ligand: Protein A

Bead Matrix: Magnetic particles

Bead Size: 40 µm

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1 ml settled Beads (4 ml 25% slurry)

Storage: 4 °C

Storage Comment: Store at 4°C, do NOT freeze.

Expiry Date: 12 months

References

Li, Wang, Gao, Li: "Identification and characterisation of the anti-oxidative stress properties of the lamprey prohibitin 2 gene." in: Fish & shellfish immunology, Vol. 42, Issue 2, pp. 447-56, (2015) (PubMed).

Lu, Shi, Chen, Wang: "The regulation of HanA during heterocyst development in cyanobacterium Anabaena sp. PCC 7120." in: World journal of microbiology & biotechnology, Vol. 30, Issue 10, pp. 2673-80, (2014) (PubMed).

Wang, Xu, Xu, Zhao, Wang: "Collaboration between a soluble C-type lectin and calreticulin facilitates white spot syndrome virus infection in shrimp." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2106-17, (2014) (PubMed).

Pang, Xiao, Liu, Li: "Identification and characterization of the lamprey high-mobility group box 1 gene." in: PLoS ONE, Vol. 7, Issue 4, pp. e35755, (2012) (PubMed).

Noberini, Rubio de la Torre, Pasquale: "Profiling Eph receptor expression in cells and tissues: a targeted mass spectrometry approach." in: Cell adhesion & migration, Vol. 6, Issue 2, pp. 102-12, (2012) (PubMed).

Dai, Zhang, Tian, Zhang: "Expression profile of HMBOX1, a novel transcription factor, in human cancers using highly specific monoclonal antibodies." in: Experimental and therapeutic medicine, Vol. 2, Issue 3, pp. 487-490, (2012) (PubMed).

Target Details

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein Bead, repB Bead

Background: Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs, mainly through Fc regions. Native Protein A has five IgG binding domains and many unknown-function repeated sequences. Recombinant Protein A only contains five high-affinity IgG binding domains to reduce nonspecific binding.