MagSi-IVD Beads Trial Kit

Catalog No. ABIN1721074

Product Details

Target: Streptavidin

Application: Separation (Sep)

Purpose: This kit includes 1 mL of the 8 different MagSi-STA products and is intended for evaluation purposes during trial phase of developing new assays, or bead replacement in existing assays.

Specificity: Some IVD assays have requirements which are not covered by standard products in the market. The purpose of this kit is to find the best candidate for your assay application. However, if the products in this kit do not meet the requirements completely, we offer further customization on specific parameters. The initial evaluation of this MagSi-IVD trial kit will serve as a starting point for product optimization by our product development team. Our development of magnetic beads for IVD-use is focused on optimizing all relevant physical/chemical parameters. This customization approach will make your assay development more efficient, faster and cheaper.

Examples of product optimization:
Parameter: Product optimization options
Dynamic binding capacity (mean / variation): Coupling chemistry
Suspension time: Size / Magnetic content / storage buffer
Separation speed: Size / Magnetic content / storage buffer
Non-specific interaction: Adjusting functionalized surface / blocking agent
Optical density: Size

Characteristics: MagSi-STA beads are superparamagnetic spherical beads with a large surface made up by a nano-porous structure. The streptavidin coated beads are designed for any type of IVD sandwich assay (chemiluminescence-, fluorescence- or colorimetry-based) and can be applied on any IVD analyser.

Binding of molecules onto MagSi-STA is based on the strong non-covalent interaction between streptavidin and biotin (Ka = 1015 M-1). Biotin is easily conjugated to various molecules and inexpensive biotinylated products are sold by many companies. MagSi-STA beads are coated with recombinant streptavidin (53 kDa) with shortened N- and C-terminus for improved solubility and accessibility of the sites. Albumin binding sites are eliminated for optimal specificity.

All MagSi beads included in this kit consist of a silica matrix, which does not have luminescent properties like for instance polystyrene. This results in comparatively low background signals in chemiluminescence- and UV/Vis read outs and enhances the assay sensitivity and dynamic range. Therefore, MagSi-STA beads are suitable for any assay, regardless of read out method used. The silica matrix ensures product stability over a wide pH range and at high ionic strength conditions as well.

Our production process has been developed for a very flexible but controlled magnetic bead synthesis and streptavidin coating. This process enables MagnaMedics to set the range for biotin binding capacity as narrow as needed (±10%). For some application this is important, because the parameters in play, like bead concentration, total amount of beads and binding kinetics of biotinylated targets, can be set as fixed values in optimizing and validating an IVD assay.

The streptavidin surface coating can be controlled with low variation for a broad range of desired binding capacities (up to 8000 pmol free biotin/mg beads, with ±10%) to ensure optimal reproducibility for your applications.

Components: This kit includes 1 ml of the 8 different MagSi-STA products

Material not included: Mixer/vortex to mix samples and re-suspend bead
Magnetic separator

Bead Ligand: Streptavidin

Bead Matrix: Magnetic Silica particles

Bead Size: Bead size: 600 nm,1 µm,3 µm

Application Details

Application Notes: MagSi-STA
It is strongly recommended to test all beads in parallel. The result of this will either define the right product immediately or function as a guide towards a suitable product for the application (see also

Comment:

Bead size
Three bead sizes are included in our kit: 600 nm, 1.0 µm and 3 µm. The sedimentation time of 600nm MagSi beads has been optimized and is approx. 4 times longer compared to 1.0µm beads. This allows e.g. long incubation times without shaking/mixing etc. MagSi beads with a diameter of 3µm have much stronger magnetic properties and will separate much faster compared to 600nm in the same conditions.

Surface activation
Two types of surface activation for streptavidin coating are included in our kit . Carboxyl-activation is used for higher binding capacities of MagSi-STA beads, Tosyl-activation is typically used for lower binding capacities. Tosyl-activated beads are also more hydrophobic than carboxyl-activated beads. Because of differences in hydrophobicity, the type of surface activation may also influence the sensitivity of the assay.

Free biotin binding capacity
The optimal binding capacity of streptavidin coated magnetic beads is dependent on the sensitivity requirements of an assay. In this kit, beads with different binding capacities are included, covering the total range of free biotin binding capacities of competitor beads currently used in the market, and even more. It is intended to find the bead with the optimal binding capacity for your specific application.

Protocol: MagSi-STA beads are supplied as a suspension containing 10 mg magnetic beads per mL, in phosphate buffer saline pH 7.4. The products contain 0.05% Tween 20 and 0.05% sodium azide as preservative. The MagSi-IVD trial kit contains 8 x 1 mL of each MagSi-STA product as described.

Product: MagSi-STA 600
Size: 600 nm
Surface activation: Carboxylated
Free biotin binding capacity: 3500-5000 pmol/mg

Product: MagSi-STA 600 BI
Size: 600 nm
Surface activation: Carboxylated
Free biotin binding capacity: 6000-6800 pmol/mg

Product: MagSi-STA 1.0 L
Size: 1.0 µm
Surface activation: Carboxylated
Free biotin binding capacity: 1200-2000 pmol/mg

Product: MagSi-STA 1.0
Size: 1.0 µm
Surface activation: Carboxylated
Free biotin binding capacity: 3500-5000 pmol/mg

Product: MagSi-STA 1.0 TL
Size: 1.0 µm
Surface activation: Tosylated
Free biotin binding capacity: 1200-2000 pmol/mg

Product: MagSi-STA 1.0 TS
Size: 1.0 µm
Surface activation: Tosylated
Free biotin binding capacity: 3500-5000 pmol/mg

Product: MagSi-STA 3.0 L
Size: 3.0 µm
Surface activation: Carboxylated
Free biotin binding capacity: 700-1200 pmol/mg

Product: MagSi-STA 3.0 TL
Size: 3.0µm
Surface activation: Tosylated
Free biotin binding capacity: 500-900 pmol/mg

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Handling Advice: Store in well closed vial and in upright position to prevent drying of the beads, because this may result in a decrease of activity.
Do not freeze the product!
Vortex well before use. Wash the beads to remove preservatives that could interfere with your test.

Storage: 4 °C

Expiry Date: 12 months

Target Details

Target: Streptavidin

Abstract: Streptavidin Products