Protein A Resin

Catalog No. ABIN1536564

Product Details

Target: Protein A

Reactivity: Staphylococcus aureus

Application: Purification (Purif), Affinity Chromatography (AC)

Purpose: Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

Characteristics: Protein A Resin can also be used for immunoprecipitation of proteins,protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Ligand: Recombinant Streptococcal protein A expressed in E. coli
Number of IgG binding sites per ligand: 5
MW of ligand: Approximately 34 kDa
PI of ligand: 5.17
Degree of substitution: Approximately 2 mg protein A/ml settled resin
Static binding capacity: >20 mg rabbit IgG/ml settled resin
Matrix: spherical agarose, 4%
Average particle size: 90 μm (45-165 μm)

Bead Ligand: Protein A

Bead Matrix: Agarose beads

Bead Size: 90 µm

Application Details

Comment:

High temperature heating is not recommended. The agarose melts above 65°C.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1X PBS containing 20% ethanol

Storage: 4 °C

Storage Comment: Store regenerated Protein A Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.

Expiry Date: 18 months

References

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Target Details

Target: Protein A

Abstract: Protein A Products

Synonyms: alpha protein Bead, repB Bead

Background: Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding, the Fab region is available for binding antigens.