Jurkat cells, a human T-cell leukemia cell line, can be characterized using specific antibody markers. The most commonly used markers for Jurkat cells include CD3, CD4, and CD45. CD3 is a T-cell receptor marker, CD4 is a co-receptor for the major histocompatibility complex class II, and CD45 is a pan-leukocyte marker. The expression of CD3 and CD4 confirms their T-cell lineage, while CD45 serves as a general leukocyte marker. These antibody markers aid in distinguishing Jurkat cells from other cell types, enabling researchers to identify and study Jurkat cells in immunological research and investigations related to T-cell biology and leukemia.