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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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604,384 results :

JAG1 antibody (Internal Region)

JAG1 Reactivity: Human, Mouse WB, ELISA, IHC, FM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Jagged 1 (JAG1) (Internal Region) antibody (ABIN1043767)

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody.

Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN129524

Datasheet as PDF

APP antibody (AA 190-290)

APP Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 190-290) antibody (ABIN3020680)

Western blot analysis of extracts of various cell lines, using APP antibody.

Immunohistochemistry of paraffin-embedded human esophagus using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human stomach using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at dilution of 1:100 (40x lens).

5 images

Independent Validation

1 validation

Catalog No. ABIN3020680

Datasheet as PDF

Histone 3 antibody (AA 71-136)

H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

K562 Cell lysates probed with Histone H3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated .

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

13 references

Catalog No. ABIN739109

Datasheet as PDF

Collagen Type I antibody (AA 1321-1400)

COL1 Reactivity: Human, Rat, Mouse, Rabbit, Dog, Fish WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS Host: Rabbit Polyclonal unconjugated

SW480 lysates probed with Rabbit Anti-Pro-Collagen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:300 followed by conjugation to the secondary PE antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse muscle lysates probed with Rabbit Anti-Pro-Collegen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

16 references

Catalog No. ABIN670386

Datasheet as PDF

CD34 antibody (AA 301-385)

CD34 Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Goat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse SP20 Myeloma Cells

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated used at 1:200 dilution for 40 minutes at 37°C

Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Antigen: 0.

6 images

21 references

Catalog No. ABIN671361

Datasheet as PDF

Fibronectin antibody (AA 1201-1300)

Reactivity: Human, Mouse, Rat, Cow WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

Image kindly submitted by Edita Aksamitiene as part of the free sample program.

Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (ABIN671646) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Image provided by One World Lab validation program.

Paraformaldehyde-fixed, paraffin embedded rat intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Downregulation of miR-27a with a miR-27a inhibitor prevented high glucose (HG)-induced mesangial cell (MC) proliferation and the expression of ECM-associated profibrotic genes via upregulation of PPARγ expression.

Inhibition of miR-27a with antagomir-27a reduces the expression of profibrotic genes and ECM proteins in the kidney glomeruli of STZ-induced diabetic rats.

7 images

13 references

Catalog No. ABIN671646

Datasheet as PDF

NRF2 antibody (pSer40)

NFE2L2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining

L1 mouse heart, L2 mouse muscle lysates probed (ABIN676673) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (ABIN676673) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

19 references

Catalog No. ABIN676673

Datasheet as PDF

PTGS2 antibody (AA 501-604)

PTGS2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated (ABIN672471) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Effects of Celecoxib on COX-2 and cyclin D1 activation in SKOV-3 xenograft tumors: (A) Western blot analysis of SKOV-3 tumors treated with a high-dose of Celecoxib (100 mg/kg).

COX protein levels in xenograft tumors of nude mice treated or not treated with combined treatment of SC-560 and celecoxib.

COX-2 over expressed in MDR-Caco-2 cells, which could be reversed by sinomenine.

9 images

14 references

Catalog No. ABIN672471

Datasheet as PDF

HIF1A antibody (AA 341-450)

HIF1A Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

HL-60 cell lysates probed with Anti-HIF-1 Alpha Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (ABIN672546), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (ABIN672546) at 1:200 in 4 °C.

KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated followed by a FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody , Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

7 images

10 references

Catalog No. ABIN672546

Datasheet as PDF

RANKL antibody (AA 221-300)

TNFSF11 Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Levels of OPG in third-generation osteoblasts following treatment with different doses of r-Mt cpn10 for 8 h.

Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-Osteoprotegerin Polyclonal Antibody (ABIN668556), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program.

Lane 1: Mouse heart lysates, Lane 2: Mouse kidney lysates, Lane 3: Mouse liver lysates probed with OPG Polyclonal Antibody at 1:300 overnight at 4˚C.

Formalin-fixed and paraffin embedded rat tibia tissue labeled with Anti-Osteoprotegerin Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Levels of OPG in third-generation osteoblasts following treatment with r-Mt cpn10 (10 μg/mL) for 2, 4, 8 and 24 h.

6 images

12 references

Catalog No. ABIN668556

Datasheet as PDF

Beclin 1 antibody (AA 201-330)

BECN1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

293T lyates probed with Rabbit Anti-Beclin 1 Polyclonal Antibody, Unconjugated at 1:300 in 4˚C overnight.

Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) at 1:200 in 4C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rabbit myocardium labeled with Anti-BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

8 images

11 references

Catalog No. ABIN675384

Datasheet as PDF

CD90 antibody (AA 31-120)

THY1 Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Sheep fat cells stained withAnti-Thy-1 CD90 Thy1.

Sheep fat cells stained withAnti-Thy-1/CD90/Thy1.

Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Mouse splenocytes probed with Rabbit Anti-Thy-1 Polyclonal Antibody, PE-Cy5.

6 images

23 references

Catalog No. ABIN728038

Datasheet as PDF

VEGF antibody (AA 27-120)

VEGF Reactivity: Human, Mouse, Rat, Pig, Goat WB, ELISA, IHC (p), IHC (fro), IF (p), FACS Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (ABIN727205) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Cells labeled with Anti-VEGF Polyclonal Antibody (ABIN727205).

HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody Alexa Fluor 488 Conjugated.

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (ABIN727205) at 1:200 in 4 °C.

6 images

14 references

Catalog No. ABIN727205

Datasheet as PDF

GFAP antibody (AA 51-150)

GFAP Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei.

RSC96 cells probed with GFAP Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation program.

Astrocytes were stained with GFAP polyclonal antibody .

Formalin-fixed and paraffin embedded mouse spinal cord stained with GFAP Polyclonal Antibody at 1:100 dilution.

8 images

20 references

Catalog No. ABIN726200

Datasheet as PDF

IL-1 beta antibody (AA 101-200)

IL1B Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro), DB Host: Rabbit Polyclonal unconjugated

Raw264.

GSPIONs do not increase expression of pro-inflammatory cytokines in lung parenchyma.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse lung labeled with (ABIN728503) Rabbit Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.

Image was kindly submitted by Dr.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

Effects of ALA on cardiac function, inflammation and autophagy in CLP-induced septic rats.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

8 images

16 references

Catalog No. ABIN728503

Datasheet as PDF

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