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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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568,044 results :

GLI3 antibody

GLI3 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

Western Blot of GLI3 in the SLC mice.

Expression of genes and proteins related to Hh signaling in SLC mice.

10 images

Independent Validation

1 validation

Catalog No. ABIN2855813

Datasheet as PDF

Histone 3 antibody (3meLys4)

H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Histone H3K4me3 antibody tested by Western blot.

Histone H3 trimethyl Lys4 antibody tested by dot blot analysis.

Detection of H3K4me3 by immunofluorescence U2OS cells were stained with H3K4me3 antibody at a dilution of 1:500.

Histone H3K4me3 antibody tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with Acute Myelocytic Leukemia (AML) cell line chromatin and 4 μg of Histone H3K4me3 antibody.

6 images

Independent Validation

1 validation

Catalog No. ABIN6971977

Datasheet as PDF

ETV4 antibody (AA 1-207)

ETV4 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunofluorescence analysis of U-2 OS cells using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Alignment tracks from CUT&RUN targeting ETV4 in mouse fore limb (11.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded Human skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

10 images

Independent Validation

1 validation

Catalog No. ABIN6140332

Datasheet as PDF

CD163 antibody (AA 1001-1121)

CD163 Reactivity: Human, Mouse, Rat, Pig, Dog WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti CD163 Polyclonal Antibody, Unconjugated (ABIN741570) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Chronic phase glibenclamide reduces inflammation and improves the macrophage phenotype in EAE.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-CD163/M130 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (ABIN741570) followed by conjugation to the secondary antibody and DAB staining

L1 rat brain, L2 mouse uterus lysates probed (ABIN741570) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C.

7 images

27 references

Catalog No. ABIN741570

Datasheet as PDF

BAX antibody (AA 84-175)

BAX Reactivity: Human, Mouse, Rat, Pig, Cow, Rabbit, Chicken, Zebrafish (Danio rerio) WB, ELISA, FACS, IP, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

L1 rat brain, L2 mouse liver lysates probed at 1:200 in 4˚C.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:800 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, unconjugated (ABIN725390) at 1: 800 followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Human HL-60 cells probed with Bax Polyclonal Antibody, Unconjugated (green) at 1:30 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Formalin-fixed and paraffin embedded rabbit brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated (ABIN725390) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Anti –BAX Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining

L1 rat brain, L2 mouse liver lysates probed (ABIN725390) at 1:200 in 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-Bax Polyclonal Antibody, Unconjugated at 1:800 followed by conjugation to the secondary antibody and DAB staining

11 images

34 references

Catalog No. ABIN725390

Datasheet as PDF

NF-kB p65 antibody (AA 51-100)

NFkBP65 Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Chicken WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (ABIN668961), Unconjugated at 1:600 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Western blot analysis of P-Akt/Akt ratio, NF-kB and P-NF-kB proteins of rats fed either placebo, (A) B.

7 images

30 references

Catalog No. ABIN668961

Datasheet as PDF

CD8 antibody (AA 51-150)

CD8 Reactivity: Human, Mouse, Rat FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD8 Polyclonal Antibody .

$Quantification and representative immunohistological staining of immune cells in the fracture haematoma/callus.$ IHC (p)

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled Anti-CD8 Polyclonal Antibody, Unconjugated (ABIN671391) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C.

Image kindly submitted by Amy Beck as part of the free sample program.

Mouse spleen lysate probed with Anti-CD8 Polyclonal Antibody at 1:5000 90min in 37˚C.

H&E and IHC staining for CD8, IL-12 and IFN-γ in subcutaneous LLC1 tumors.

7 images

26 references

Catalog No. ABIN671391

Datasheet as PDF

MAP1LC3A antibody (cleaved)

MAP1LC3A Reactivity: Human, Mouse WB, IF, IHC (p), ICC Host: Rabbit Polyclonal RB38908 unconjugated

Western blot analysis of anti-cleaved-LC3 (G8a) Pab 1805a in mouse brain tissue lysate.

Immunofluorescent analysis of 4 % paraforldehyde-fixed, 0.

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower).

Immunofluorescent analysis of 4 % paraforldehyde-fixed, 0.

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower).

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A)(R)(upper) or Beta-actin (lower).

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) Antibody 1805a (upper) or Beta-actin (lower).

7 images

35 references

Catalog No. ABIN388483

Datasheet as PDF

beta Actin antibody (AA 350-375)

ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-Beta Actin Antibody.

Immunohistochemistry of Rabbit Anti-Beta Actin Antibody.

Distinct functions of HOXD proteins in regulation of neuronal differentiation.

E2F6 is essential for HOXC9 induction of G1 arrest and repression of cell cycle genes.

Effects of TRPM2 inhibition and RNAi knockdown in cancerous and non-cancerous breast cell lines after chemotherapeutic treatments.

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

10 images

31 references

Catalog No. ABIN129657

Datasheet as PDF

HIS2AV (pSer137) antibody

Reactivity: Drosophila melanogaster ELISA, IHC, WB Host: Rabbit Polyclonal unconjugated

Western blot using affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head).

Systemic hormetic responses from muscle-specific DNA damage.

dH1 depletion induces DNA damage.

WB analyses with αdH1, αγH2Av and αH4 of increasing amounts of extracts (lanes 1-3) prepared from siRNAdH1, siRNAlacZ and untreated cells.

WB analysis with αγH2Av and αtubulin at different time points after X-ray irradiation (10Gy) of siRNAdH1 and untreated cells.

DNA damage induced by dH1 depletion associates with R-loops accumulation.

WB analyses with αdH1, αγH2Av and αH4 antibodies of siRNAdH1 and untreated cells sorted at G1-, S and G2/M-phase.

Immunostaining with αγH2Av (green) and αCaspase 3 (red) of wing imaginal discs from dH1-depleted his1RNAi flies upon dATRRNAi co-depletion (bottom) or not (top).

hth mutant nuclei show increased number of DNA breaks.

B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA.

10 images

27 references

Catalog No. ABIN129671

Datasheet as PDF

beta Actin antibody (AA 2-16)

ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle section (dilution 1 : 500; red).

7 images

3 references

Independent Validation

1 validation

Catalog No. ABIN1742508

Datasheet as PDF

Histone 3 antibody (H3K9ac)

H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Western blot of Histone H3 acetyl Lys9 antibody (pAb).

ChIP of Histone H3 acetyl Lys9 pAb.

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq.

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.

7 images

3 references

Independent Validation

1 validation

Catalog No. ABIN2668415

Datasheet as PDF

Caspase 3 antibody (AA 67-175)

CASP3 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

Anti-Caspase3 antibody, IHC(P) IHC(P): Mouse Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Rat Intestine Tissue

Anti-Caspase3 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue

Anti- Caspase3 antibody, Western blotting All lanes: Anti Caspase3 at 0.

5 images

103 references

Catalog No. ABIN3043802

Datasheet as PDF

SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody

Rockland 200-401-a50 SARS-CoV N Reactivity: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, FM, IHC Host: Rabbit Polyclonal unconjugated

Western Blot of Rabbit Anti-SARS CoV Nucleocapsid (N) Protein Antibody.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

This immunofluorescence microscopy image shows localization of SARS-CoV-2 infection in human liver ductal organoids.

Immunofluorescence of Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody.

Western blot using anti-SARS CoV Nucleocapsid (N) protein antibody shows detection of a 46-kDa band corresponding to the protein.

Western Blot of Rabbit Anti-SARS-CoV (N) Antibody tested on Caco-2 Cell Lysates before (mock), 24, and 48 hours post infection (hpi).

Immunohistochemistry results showing positive staining in placenta tissue using Anti-SARS-CoV Nucleocapsid (N) Antibody.

Immunohistochemistry of Rabbit Anti-SARS CoV Nucleocapsid Antibody.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) Protein.

Immunofluorescence of Rabbit Anti-SARS CoV (N) Protein Antibody.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein synthesis.

Immunofluorescence assay using Rabbit Anti-SARS-CoV Nucleocapsid (N) Antibody, showing viral protein detection.

ELISA results of Rabbit Anti-SARS-CoV Nucleocapsid (N) ProteinVariants.

14 images

96 references

Catalog No. ABIN6952544

Datasheet as PDF

SNAP Tag antibody

ELISA, WB, SEM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-SNAP Tag antibody (ABIN1573927)

Immunofluorescence validation image for anti-SNAP Tag antibody (ABIN1573927)

Electron Microscopy validation image for anti-SNAP Tag antibody (ABIN1573927)

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD) Lane 3: Negative 293 cell lysate Primary antibody: 1 µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (ABIN398494) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 10,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939)

4 images

3 references

Independent Validation

3 validations

Catalog No. ABIN1573927

Datasheet as PDF

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