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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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625,746 results :

NFKB1 antibody (AA 740-964)

NFKB1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 references

Independent Validation

1 validation

Catalog No. ABIN6144571

Datasheet as PDF

Complement Factor H antibody (AA 20-270)

CFH Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

1 reference

Independent Validation

1 validation

Catalog No. ABIN3023097

Datasheet as PDF

Histone 3 antibody (H3K4me)

H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat kidney using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody.

Dot Blot (DB) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody.

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody.

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse lung using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody.

23 images

4 references

Independent Validation

1 validation

Catalog No. ABIN3023251

Datasheet as PDF

Vimentin antibody (AA 371-466)

VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (ABIN672786) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (ABIN672786) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image submitted by One World Lab validation program.

20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE and transferred to PVDF membrane for western blotting analysis antibodies against protein VIM at dilution of 1:1000 with exposure time of 10 seconds.

Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated at 0.

This image was kindly submitted by Preethi Vijayaraj from UCLA, 4% PFA fixed cells, permeabilized with 0.

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

13 images

51 references

Catalog No. ABIN672786

Datasheet as PDF

Caspase 3 antibody (AA 60-100)

CASP3 Reactivity: Human, Mouse, Rat, Cow, Goat WB, IF (cc) Host: Rabbit Polyclonal unconjugated

Rat lung lysate 30ug, probed at 1:200 overnight in 4˚C.

Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (ABIN724625) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI staining

Human HepG2 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Neuronal apoptosis in acNTS of CRS mice.

Erianin induced mitochondrial apoptosis in liver cancer cells.

7 images

86 references

Catalog No. ABIN724625

Datasheet as PDF

Collagen Type I antibody

COL1 Reactivity: Human ELISA Host: Goat Polyclonal unconjugated

Decreases in the levels of activated HSC markers, SOX9, COL1 and phosphoMyl9 (PMYL9) following PAK-1 abrogation by siRNA1 in activated rat HSCs relative to their respective scrambled control levels.

Characterisation of COL1 expression in control and Sox9-null animals following liver fibrosis induction using ABIN135046.

Activated mouse HSCs (‘Control') show decreased protein levels for α-SMA, COL1 and SOX9 by immunoblotting following the loss of integrin beta-1 (‘Itgb1-null').

Elevated collagen levels in skeletal muscles in SGCA- and SGCD-null mice and in the heart of SGCD-null mice investigated using ABIN135046: Immunofluorescent images of skeletal muscles stained with collagen type I (fibrotic marker, green) and laminin (extracellular matrix of muscle fibres, red) Scale bar: 100 μm.

Immunofluorescence images of the heart stained with collagen type I (fibrotic marker, green) using ABIN135046.

(A) Effects on collagen deposition in indirect (inserts) and direct coculture of human dermal fibroblasts and siRNA-treated THP1 cells.

Immunoblot analysis of primary AF and NP cell lysates from two independent donors, D1 (P0) and D3 (P1), respectively, for COL1A1 (ABIN135046) and COL2A1 (ABIN135048).

Immunoblot Analysis using ABIN135046.

8 images

69 references

Catalog No. ABIN135046

Datasheet as PDF

GLI2 antibody (Middle Region)

GLI2 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

Immunohistochemistry validation image for anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody (ABIN2777474)

In Fig.

Host: Rabbit Target Name: GLI2 Sample Type: Jurkat Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2.

WB Suggested Anti-GLI2 Antibody Titration: 1.

Sample Type : Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop Primary Antibody Dilution : 1:50 Secondary Antibody : Goat anti-rabbit-cy3 Secondary Antibody Dilution : 1:1500 Color/Signal Descriptions : GLI2: Green Neural crest cells:: Red Gene Name : GLI2 Submitted by : Anonymous

Host: Rabbit Target Name: GLI2 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Heart Cellular Data: Myocardial cells Antibody Concentration: 4.

Rabbit Anti-GLI2 Antibody Paraffin Embedded Tissue: Human Intestine Cellular Data: Epithelial cells of intestinal gland Antibody Concentration: 4.

9 images

23 references

Independent Validation

2 validations

Catalog No. ABIN2777474

Datasheet as PDF

Histone 3 antibody (H3K27ac)

H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K27ac) antibody (ABIN2668475)

Histone H3 acetyl Lys27 antibody tested by Western blot.

Histone H3 acetyl Lys27 antibody (pAb) tested by ChIP-chip.

Histone H3 acetyl Lys27 antibody tested by dot blot analysis.

Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis.

Histone H3 acetyl Lys27 antibody tested by immunofluorescence.

7 images

23 references

Independent Validation

1 validation

Catalog No. ABIN2668475

Datasheet as PDF

Reelin antibody (AA 3143-3461)

RELN Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Reelin (RELN) (AA 3143-3461) antibody (ABIN7435136)

Detection of RELN in Mouse Fetal rat lysate using Polyclonal Antibody to Reelin (RELN)

Detection of Recombinant RL, Mouse using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Liver Tissue using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Cerebrum Tissue using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Serum using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Human HepG2 cell lysate using Polyclonal Antibody to Reelin (RELN)

Detection of RELN in Mouse Cerebrum lysate using Polyclonal Antibody to Reelin (RELN)

8 images

Independent Validation

1 validation

Catalog No. ABIN7435136

Datasheet as PDF

PI3K p85 alpha/gamma antibody (pTyr199, pTyr467)

PI3K p85a/g Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g) (pTyr199), (pTyr467) antibody (ABIN744743)

Image provided by Independent Validation Program, badge 29801.

Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin-embedded rat heart labeled with Anti-phospho-PI3K p85(Tyr467) /p55(Tyr199) Polyclonal Antibody, Unconjugated (ABIN744743) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

5 images

3 references

Independent Validation

1 validation

Catalog No. ABIN744743

Datasheet as PDF

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