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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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625,524 results :

JAG1 antibody (Internal Region)

JAG1 Reactivity: Human, Mouse WB, ELISA, IHC, FM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Jagged 1 (JAG1) (Internal Region) antibody (ABIN1043767)

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).

Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody.

Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN129524

Datasheet as PDF

APP antibody (AA 190-290)

APP Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 190-290) antibody (ABIN3020680)

Western blot analysis of extracts of various cell lines, using APP antibody.

Immunohistochemistry of paraffin-embedded human esophagus using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human stomach using APP antibody (ABIN3020679, ABIN3020680, ABIN3020681 and ABIN6213663) at dilution of 1:100 (40x lens).

5 images

Independent Validation

1 validation

Catalog No. ABIN3020680

Datasheet as PDF

GAPDH antibody (AA 1-335)

GAPDH Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting (WB) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody (ABIN678458)

Paraformaldehyde-fixed, paraffin embedded Mouse brain Antigen retrieval by boiling in sodium citrate buffer (pH6.

Mouse muscle lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

U251 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Lane 1: Rat muscle lysates Lane 2: Rat heart lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Lane 1: Hela lysates Lane 2: MCF-7 lysates probed with GAPDH Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Western blot analysis of SOX2, NANOG, and OCT protein.

7 images

16 references

Catalog No. ABIN678458

Datasheet as PDF

Histone 3 antibody (AA 71-136)

H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

K562 Cell lysates probed with Histone H3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated .

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

13 references

Catalog No. ABIN739109

Datasheet as PDF

Collagen Type I antibody (AA 1321-1400)

COL1 Reactivity: Human, Rat, Mouse, Rabbit, Dog, Fish WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS Host: Rabbit Polyclonal unconjugated

SW480 lysates probed with Rabbit Anti-Pro-Collagen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:300 followed by conjugation to the secondary PE antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse muscle lysates probed with Rabbit Anti-Pro-Collegen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

16 references

Catalog No. ABIN670386

Datasheet as PDF

CD34 antibody (AA 301-385)

CD34 Reactivity: Human, Mouse, Rat, Rabbit, Goat, Chicken FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse SP20 Myeloma Cells

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated used at 1:200 dilution for 40 minutes at 37°C

Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Antigen: 0.

6 images

21 references

Catalog No. ABIN671361

Datasheet as PDF

Fibronectin antibody (AA 1201-1300)

Reactivity: Human, Mouse, Rat, Cow WB, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Host: Rabbit Polyclonal unconjugated

Image kindly submitted by Edita Aksamitiene as part of the free sample program.

Formalin-fixed and rat pancreas tissue labeled with Anti-FN/Fibronectin 1 Polyclonal Antibody, Unconjugated (ABIN671646) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated

Paraformaldehyde-fixed, paraffin embedded human kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Image provided by One World Lab validation program.

Paraformaldehyde-fixed, paraffin embedded rat intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Fibronectin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Downregulation of miR-27a with a miR-27a inhibitor prevented high glucose (HG)-induced mesangial cell (MC) proliferation and the expression of ECM-associated profibrotic genes via upregulation of PPARγ expression.

Inhibition of miR-27a with antagomir-27a reduces the expression of profibrotic genes and ECM proteins in the kidney glomeruli of STZ-induced diabetic rats.

7 images

13 references

Catalog No. ABIN671646

Datasheet as PDF

NRF2 antibody (pSer40)

NFE2L2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining

L1 mouse heart, L2 mouse muscle lysates probed (ABIN676673) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (ABIN676673) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

19 references

Catalog No. ABIN676673

Datasheet as PDF

PTGS2 antibody (AA 501-604)

PTGS2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated (ABIN672471) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Effects of Celecoxib on COX-2 and cyclin D1 activation in SKOV-3 xenograft tumors: (A) Western blot analysis of SKOV-3 tumors treated with a high-dose of Celecoxib (100 mg/kg).

COX protein levels in xenograft tumors of nude mice treated or not treated with combined treatment of SC-560 and celecoxib.

COX-2 over expressed in MDR-Caco-2 cells, which could be reversed by sinomenine.

9 images

14 references

Catalog No. ABIN672471

Datasheet as PDF

HIF1A antibody (AA 341-450)

HIF1A Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

HL-60 cell lysates probed with Anti-HIF-1 Alpha Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (ABIN672546), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (ABIN672546) at 1:200 in 4 °C.

KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated followed by a FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody , Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

7 images

10 references

Catalog No. ABIN672546

Datasheet as PDF

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