Multiplex IHC Antibodies
Multiplex immunohistochemistry (IHC) is a powerful tool that allows researchers to gain deeper insights into complex biological processes and disease mechanisms. Simultaneous analysis of multiple protein markers in the same tissue sample helps in understanding the spatial relationships between different cell phenotypes in situ. This spatial profiling can increase the discovery rate of new biomarkers and therapeutically targetable pathways.
Multiplex IHC Antibodies
antibodies-online offers a selection of high-qualiy antibodies suitable for multiplex IHC experiments. They are thoroughly tested through our Independant Validation Initiative (IVI).
Carrier-Free Formulations for Antibodies
You want to develop your own multiplex IHC Antibodies? Discover our widge range of carrier-free IHC antibodies suitable for conjugation down below. If you have any question or need help with the selection dont hesitate to get in touch with our cutomer service.
Customarily, commercially available antibodies are provided in a solution containing phosphate buffered saline (PBS) along with various additives such as bovine serum albumin (BSA), sodium azide, and glycerol. These additives play a crucial role in preventing contamination, preserving the antibody, and ensuring stability. While these stabilizers do not generally interfere with immunodetection techniques like immunohistochemistry, ELISA, and Western Blot, they may impede the conjugation of antibodies with fluorophores, metals, and enzymes.
BSA, which functions as a protein stabilizer, can hinder conjugation efficiency by competing with the primary antibody to bind with the desired label. Sodium azide acts as a preservative but can be toxic to cells and also interfere with conjugation. Consequently, if these antibodies are to be utilized in bioconjugation techniques, it becomes necessary to eliminate some or all of these additives from the antibody solution.
The demand for carrier-free antibodies has risen due to the increasing importance of fluorescent multiplexing and advanced mass cytometry in areas such as cancer research, translational research, and drug discovery. At antibodies-online, we now provide an extensive range of high-quality antibodies that are customizable and can be tailored to meet your specific requirements.
What is Multiplex IHC?
Multiplex IHC is a technique used in biomedical research and pathology to detect and visualize multiple protein markers simultaneously in tissue samples. Immunohistochemistry is a method that allows researchers to identify specific proteins or antigens within cells or tissues using antibodies that bind to the target proteins.
What is the Difference Between Standard and Multiplex IHC?
In traditional IHC, a single antibody is used to detect one protein of interest. However, with multiplex IHC, multiple antibodies can be used simultaneously, each labeled with a different fluorophore or enzyme, to detect and distinguish multiple proteins within the same tissue section.
What are Advantages of Multiplex IHC Staining?
Multiplex IHC offers several advantages over traditional single-marker IHC. It allows researchers to study complex cellular interactions and spatial relationships between different proteins within tissues. It can provide a more comprehensive and detailed understanding of cellular processes, such as cell signaling pathways, immune responses, and tumor microenvironments (TME).
Murine frozen coronal olfactory bulb section labeled with anti-cortactin antibody ABIN2854674 (green; conj. to ATTO 550). Gri2b was labeled with ABIN5611338 (red; conj. to ATTO 550).
Spatial Analysis of Tumor Microenvironment (TME)
Multiplex IHC plays a crucial role in TME research, offering key advantages for studying the complex interactions within this dynamic ecosystem. The TME is characterized by the presence of diverse cell populations and signaling molecules. Key TME cell phenotypes are defined by the simultaneous detection of more than two markers. Multiplex IHC excels in this task, providing a comprehensive view of the spatial distribution and interactions of different cell types and their associated biomarkers.
Assessing spatial heterogeneity: Tumors are known to exhibit spatial heterogeneity, with different cell populations and signaling gradients varying across regions. Multiplex IHC enables the assessment of spatial relationships and co-localization patterns between different markers within the TME. Ultimatively Multiplex IHC helps to unravel the complex cellular landscape of the TME.
What are Tips for Multiplex IHC Staining?
- To prevent cross-reaction of secondary systems in indirect IHC methods, it is preferable to use primary antibodies that come from different host species.
- In cases where monoclonal antibodies of the same host species are used but with different isotypes, their detection can be achieved by employing isotype-specific secondary antibodies.
- In chromogenic IHC, if only primary antibodies from the same species and isotype are available, heat or low pH-buffers can be utilized to strip antibodies from the initial IHC round.
