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Independently validated Antibodies

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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247 results :

GLI3 antibody

GLI3 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

Cleavage Under Targets and Release Using Nuclease validation image for anti-GLI Family Zinc Finger 3 (GLI3) antibody (ABIN2855813)

WB Image GLI3 antibody detects GLI3 protein by Western blot analysis.

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3, antibody at 1:500 dilution.

IHC-P Image GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE antibody diluted at 1:1000

Expression changes of Hedgehog signaling components.

Western Blot of GLI3 in the SLC mice.

Expression of genes and proteins related to Hh signaling in SLC mice.

10 images

Independent Validation

1 validation

Catalog No. ABIN2855813

Datasheet as PDF

NFKB1 antibody (AA 860-969)

NFKB1 Reactivity: Human WB, ELISA, IF, IHC (p), PLA Host: Mouse Monoclonal 4A11 unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibody (ABIN561981)

Western Blot detection against Immunogen (37.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.

Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon.

Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1.

Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell.

8 images

Independent Validation

1 validation

Catalog No. ABIN561981

Datasheet as PDF

Histone 3 antibody (3meLys4)

H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (3meLys4) antibody (ABIN6971977)

Histone H3K4me3 antibody tested by Western blot.

Histone H3 trimethyl Lys4 antibody tested by dot blot analysis.

Detection of H3K4me3 by immunofluorescence U2OS cells were stained with H3K4me3 antibody at a dilution of 1:500.

Histone H3K4me3 antibody tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with Acute Myelocytic Leukemia (AML) cell line chromatin and 4 μg of Histone H3K4me3 antibody.

6 images

Independent Validation

1 validation

Catalog No. ABIN6971977

Datasheet as PDF

ETV4 antibody (AA 1-207)

ETV4 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Ets Variant 4 (ETV4) (AA 1-207) antibody (ABIN6131217)

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunofluorescence analysis of U-2 OS cells using Pea3 / ETV4 Rabbit pAb (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Alignment tracks from CUT&RUN targeting ETV4 in mouse fore limb (11.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded Human skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse skin using Pea3 / ETV4 antibody (ABIN6131217, ABIN6140332, ABIN6140334 and ABIN6221480) at dilution of 1:100 (40x lens).

10 images

Independent Validation

1 validation

Catalog No. ABIN6140332

Datasheet as PDF

beta Actin antibody (AA 2-16)

ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting validation image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Western Blotting (WB) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunocytochemistry (ICC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Immunohistochemistry (IHC) image for anti-Actin, beta (ACTB) (AA 2-16) antibody (ABIN1742508)

Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse muscle section (dilution 1 : 500; red).

7 images

3 references

Independent Validation

1 validation

Catalog No. ABIN1742508

Datasheet as PDF

Histone 3 antibody (H3K36me3)

H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K36me3) antibody (ABIN2668403)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K36me3) antibody (ABIN2668403)

Western blot of Histone H3 trimethyl Lys36 antibody.

Dot blot of Histone H3 trimethyl Lys36 antibody.

Histone H3K36me3 antibody (mAb) tested by ChIP-Seq.

Histone H3 dimethyl Lys36 antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Lys36 antibody tested by Western blot.

7 images

4 references

Independent Validation

1 validation

Catalog No. ABIN2668403

Datasheet as PDF

Histone 3 antibody (H3K9ac)

H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K9ac) antibody (ABIN2667854)

Western blot of Histone H3 acetyl Lys9 antibody (pAb).

ChIP of Histone H3 acetyl Lys9 pAb.

Histone H3 acetyl Lys9 antibody tested by dot blot analysis.

Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq.

Immunofluorescence stain of Histone H3 acetyl Lys9 pAb.

7 images

3 references

Independent Validation

1 validation

Catalog No. ABIN2668415

Datasheet as PDF

SNAP Tag antibody

ELISA, WB, SEM Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-SNAP Tag antibody (ABIN1573927)

Immunofluorescence validation image for anti-SNAP Tag antibody (ABIN1573927)

Electron Microscopy validation image for anti-SNAP Tag antibody (ABIN1573927)

Lane 1-2: SNAP fusion protein in 293 cell lysate (~ 28 kD) Lane 3: Negative 293 cell lysate Primary antibody: 1 µg/mL Rabbit Anti-SNAP-tag Polyclonal Antibody (ABIN398494) Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (ABIN398323, 1: 10,000) The signal was developed with LumiSensorTM HRP Substrate Kit (ABIN769939)

4 images

3 references

Independent Validation

3 validations

Catalog No. ABIN1573927

Datasheet as PDF

MTOR antibody (AA 2436-2492)

mTOR Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody (ABIN676403)

Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated and DAPI staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (ABIN676403) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

9 references

Independent Validation

1 validation

Catalog No. ABIN676403

Datasheet as PDF

Cadherin antibody (AA 841-882)

Reactivity: Human, Mouse WB, FACS, IF (cc), IF (p), IHC (p), ELISA, IHC (fro), ICC Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Flow Cytometry validation image for anti-Cadherin (AA 841-882) antibody (ABIN687682)

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN687682) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN687682

Datasheet as PDF

TTF1 antibody (AA 201-300)

TTF1 Reactivity: Human, Rat, Mouse WB, FACS, IF (cc), ICC Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody (ABIN728713)

Human A549 probed with TTF1 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Independently Validated Antibody, image provided by Science Direct, badge number 028752.

PFA fixed A549 cells with Anti-TTF1 Polyclonal Antibody at 1:200 dilution, followed by conjugation to the secondary, Goat Anti-Rabbit IgG A488 1:100 for 30min.

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN728713

Datasheet as PDF

IGF1R antibody (AA 251-350)

IGF1R Reactivity: Human, Mouse, Rat, Dog FACS Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 251-350) antibody (ABIN726575)

Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD221 Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

4 images

2 references

Independent Validation

1 validation

Catalog No. ABIN726575

Datasheet as PDF

FZD7 antibody (AA 501-574)

FZD7 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Frizzled Family Receptor 7 (FZD7) (AA 501-574) antibody (ABIN710051)

Image provided by the Independent Validation Program (badge number 29805).

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Frizzled 7/FZE3 Polyclonal Antibody, Unconjugated (ABIN710051) at 1:200, followed by conjugation to the secondary antibody and DAB staining

4 images

1 reference

Independent Validation

1 validation

Catalog No. ABIN710051

Datasheet as PDF

RUNX3 antibody (AA 31-130)

RUNX3 Reactivity: Human WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Immunofluorescence validation image for anti-Runt-Related Transcription Factor 3 (RUNX3) (AA 31-130) antibody (ABIN1714069)

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Runx3 Polyclonal Antibody, Unconjugated (ABIN1714069) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5 images

Independent Validation

1 validation

Catalog No. ABIN1714069

Datasheet as PDF

CDH6 antibody (AA 281-380)

CDH6 Reactivity: Human WB, ELISA, FACS Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

Flow Cytometry validation image for anti-K-Cadherin (CDH6) (AA 281-380) antibody (ABIN715286)

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti K Cadherin Polyclonal Antibody, Unconjugated (ABIN715286) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Images provided by the Independent Validation Program badge number 29743.

Formalin-fixed and paraffin embedded rat brain Rabbit Anti K Cadherin Polyclonal Antibody, Unconjugated (ABIN715286) at 1:200 followed by conjugation to the secondary antibody and DAB staining

5 images

Independent Validation

1 validation

Catalog No. ABIN715286

Datasheet as PDF

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