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Independently validated Antibodies

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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244 results :

RFP antibody

RFP Reactivity: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

356 references

Independent Validation

1 validation

Catalog No. ABIN129578

Datasheet as PDF

Collagen Type I antibody

COL1 Reactivity: Human, Rat, Mouse, Cow, Mammalian WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Immunohistochemistry validation image for anti-Collagen, Type I (COL1) antibody (ABIN5596819)

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunofluorescence staining of extracellular and intracellular type I collagen.

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

46 images

62 references

Independent Validation

2 validations

Catalog No. ABIN5596819

Datasheet as PDF

DYKDDDDK Tag antibody

WB, ELISA, IP Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

8 images

20 references

Independent Validation

2 validations

Catalog No. ABIN99294

Datasheet as PDF

E-cadherin antibody (AA 401-500)

CDH1 Reactivity: Human, Mouse, Rat WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.

MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

Image provided by One World Lab validation program.

Image provided by Independent Validation badge 29768.

Image provided by One World Lab validation program.

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

14 images

14 references

Independent Validation

1 validation

Catalog No. ABIN1387847

Datasheet as PDF

Caspase 8 antibody (AA 411-482)

CASP8 Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 references

Independent Validation

1 validation

Catalog No. ABIN724205

Datasheet as PDF

TNF alpha antibody (AA 181-235)

TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Histology and immunofluorescence in rat corneal sections.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 images

21 references

Independent Validation

1 validation

Catalog No. ABIN677318

Datasheet as PDF

Dendra 2 antibody

Reactivity: Coral WB, FACS, ICC Host: Rabbit Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct, reducing and non-reducing conditions.

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

8 images

16 references

Independent Validation

1 validation

Catalog No. ABIN361314

Datasheet as PDF

CD63 antibody (AA 120-175)

CD63 Reactivity: Human, Mouse, Rat, Monkey, Dog FACS, WB, IHC, IF Host: Goat Polyclonal unconjugated

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Anti-CD63 Ab at 1/1,000 dilution; Iysate at 50 µg per lane; Rabbit polyclonal to goat lgG (HRP) at 1/10,000 dilution

Immunofluorescence — anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;

Immuno-localization of CD63 in the hen reproductive trac

Representative immunogold labeling (using TEM) images of CSF-derived EVs from control and prenatally cocaine-exposed male and female monkeys are shown.

Flow cytometry analysis of membrane CD63 level in PMN of control and SCK cows.

Expression of exosomes in bronchoalveolar lavage in Rat BAL fluid Source: PMID36035371

Expression of exosomes in Rat lung tissue Source: PMID36035371

Expression of exosomes in Rat Brain Tissue Source: PMID36035371

Immunofluorescence (IF) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

18 images

10 references

Independent Validation

3 validations

Catalog No. ABIN1440014

Datasheet as PDF

Iba1 antibody (C-Term)

IBA1 Reactivity: Human WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

Independent Validation

1 validation

Catalog No. ABIN2857032

Datasheet as PDF

SOX2 antibody

KO Validated SOX2 Reactivity: Human WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2, antibody at 1:100 dilution.

IP Image Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] , or SOX2 antibody [GT1876], Western blot analysis was performed using SOX2 antibody [N1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2, antibody at 1:160 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2, antibody at 1:80 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.

FACS Image Flow cytometry on human embryonic stem cells, staining with SOX2 ,antibody at 1:100 dilution(purple) or rabbit IgG (black).

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

Western blot analysis of CD44, octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), SOX9, transketolase (TKT), hypoxia-inducible factor 1 alpha (HIF-α) and phosphoglycerate kinase 1 (PGK-1) in CSC-like and non-CSC UM1 cells (A).

KD of Cops2, but not any other CSN subunits, reduces the expression of Nanog and Oct4.

In vitro characterizations of hc-iPSCs.

S1PR1 activation enhanced NSCs proliferation and neuronal differentiation in hippocampal dentate gyrus (DG) after TBI.

S1PR1-induced NSCs proliferation and neuronal differentiation in TBI rats were affected by MEK/Erk activity.

17 images

11 references

Independent Validation

1 validation

Catalog No. ABIN2855074

Datasheet as PDF

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