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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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73,009 results :

Transferrin Receptor antibody

TFRC Reactivity: Human FACS, IP, ICC Host: Mouse Monoclonal MEM-75 unconjugated

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE .

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green).

Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75, green).

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

6 images

1 reference

Catalog No. ABIN94221

Datasheet as PDF

Cytokeratin 18 antibody

KRT18 Reactivity: Human WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal DC-10 unconjugated

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Detectionof cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10 .

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 ((ABIN94282), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

6 images

4 references

Catalog No. ABIN94282

Datasheet as PDF

CD247 antibody (Tyr72)

CD247 Reactivity: Human, Mouse WB, FACS, ICC Host: Mouse Monoclonal EM-26 unconjugated

Anti-Hu CD3 zeta (pY72) Purified (clone EM-26) specificity verification by WB.

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants.

Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26, light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) works in Flow Cytometry application Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol.

Separation of pervanadate stimulated Jurkat cell suspension stained using anti-human CD3 zeta (pY72) (EM-26) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from pervanadate stimulated Jurkat cell suspension unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

6 images

1 reference

Catalog No. ABIN2749060

Datasheet as PDF

Integrin beta 2 antibody

ITGB2 Reactivity: Human FACS, WB, IP, IHC (fro), Func, IHC (p) Host: Mouse Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 images

5 references

Catalog No. ABIN2749019

Datasheet as PDF

Cytokeratin 19 antibody

KRT19 Reactivity: Human WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal A53-B-A2 unconjugated

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (ABIN94290, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions.

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

6 images

2 references

Catalog No. ABIN94290

Datasheet as PDF

CD63 antibody

CD63 Reactivity: Human FACS, IHC (p), IP, ICC Host: Mouse Monoclonal MEM-259 unconjugated

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259, green) after co-incubation of living cells with human Transferrin - Dyomics 547 (cat.

Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259, green).

Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259, green).

9 images

3 references

Catalog No. ABIN94214

Datasheet as PDF

Cytokeratin 18 antibody

KRT18 Reactivity: Mammalian WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal C-04 unconjugated

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) Fig.

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 () FITC.

Flow cytometry intracellular staining pattern of HeLa cell suspension stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/ml) GAM FITC.

Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/mL, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

8 images

4 references

Catalog No. ABIN94279

Datasheet as PDF

ZAP70 antibody (C-Term)

ZAP70 Reactivity: Human WB, FACS, ICC Host: Mouse Monoclonal ZAP-03 unconjugated

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 ; 0.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western blotting analysis of human ZAP70 using mouse monoclonal antibody ZAP-03 on lysates of Jurkat cell line and HEK-293T cell line (negative control) under reducing and non-reducing conditions.

6 images

1 reference

Catalog No. ABIN125756

Datasheet as PDF

Vimentin antibody

VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunofluorescence staining (rat basophils)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

9 images

4 references

Catalog No. ABIN94491

Datasheet as PDF

SIT1 antibody

SIT1 Reactivity: Human WB, IP, FACS Host: Mouse Monoclonal SIT-01 unconjugated

Western blot of human Jurkat T cell line

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions.

Anti-SIT Purified (clone SIT-01) works in WB application.

Anti-SIT Purified (clone SIT-01) specificity verification by WB.

7 images

2 references

Catalog No. ABIN94465

Datasheet as PDF

TUBG1 antibody (C-Term)

TUBG1 Reactivity: Human, Rat, Mouse, Cow, Chicken, Plant, Protozoa, Pig WB, ICC, FACS Host: Mouse Monoclonal TU-30 unconjugated

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions.

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Fig.

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (; direct conjugate with Dyomics 547, red).

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red).

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts.

8 images

8 references

Catalog No. ABIN94313

Datasheet as PDF

Growth Hormone Receptor antibody (AA 101-200)

GHR Reactivity: Human, Mouse, Rat, Pig, Sheep WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Evaluation of the pig GHR transcript with a 4bp insert in vitro.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (ABIN671481) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated .

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Knockout of the pig GHR gene in F2 homozygous pigs (GHR4bp/4bp refers to -/-).

6 images

7 references

Catalog No. ABIN671481

Datasheet as PDF

HMGB1 antibody (AA 75-170)

HMGB1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated .

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

7 references

Catalog No. ABIN671616

Datasheet as PDF

beta Catenin antibody (pSer33, pSer37)

CATNB Reactivity: Human, Mouse, Rat WB, FACS, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon cancer tissue labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

7 images

1 reference

Catalog No. ABIN683893

Datasheet as PDF

CD28 antibody (AA 1-120)

CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded Human stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

1 reference

Catalog No. ABIN674604

Datasheet as PDF

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