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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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77,152 results :

ICAM1 antibody

ICAM1 Reactivity: Human, Rat, Cow WB, FACS, ELISA, IHC (p), ICC Host: Mouse Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions.

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111.

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

6 images

7 references

Catalog No. ABIN94177

Datasheet as PDF

Cytokeratin 19 antibody

KRT19 Reactivity: Human WB, ELISA, FACS, IHC (p), IP, ICC Host: Mouse Monoclonal A53-B-A2 unconjugated

Western Blotting analysis (reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line.

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (ABIN94290, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions.

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 μg/mL, GAM APC).

6 images

2 references

Catalog No. ABIN94290

Datasheet as PDF

CD63 antibody

CD63 Reactivity: Human FACS, IHC (p), IP, ICC Host: Mouse Monoclonal MEM-259 unconjugated

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 FITC.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD63 (TEM-259).

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 ; green) after co-incubation of living cells with human Transferrin - Dyomics 547; cell nuclei stained with DAPI (blue).

Flow cytometry analysis of IgE-activated peripheral blood stained with anti-human CD63 (MEM-259) purified, GAM-APC.

Immunocytochemistry staining of human skin fibroblasts with anti-CD63 (MEM-259, green) after co-incubation of living cells with human Transferrin - Dyomics 547 (cat.

Immunocytochemistry staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259, green).

Immunocytochemistry staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259, green).

9 images

3 references

Catalog No. ABIN94214

Datasheet as PDF

Cytokeratin 18 antibody

KRT18 Reactivity: Mammalian WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal C-04 unconjugated

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Immunohistochemistry of paraffin-embedded sections (kidney) Immunohistochemistry staining of human kidney (paraffin-embedded sections) Fig.

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 () FITC.

Flow cytometry intracellular staining pattern of HeLa cell suspension stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/ml) GAM FITC.

Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0,6 μg/mL, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

8 images

4 references

Catalog No. ABIN94279

Datasheet as PDF

ZAP70 antibody (C-Term)

ZAP70 Reactivity: Human WB, FACS, ICC Host: Mouse Monoclonal ZAP-03 unconjugated

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP-70 ; 0.

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Western blotting analysis of human ZAP70 using mouse monoclonal antibody ZAP-03 on lysates of Jurkat cell line and HEK-293T cell line (negative control) under reducing and non-reducing conditions.

6 images

1 reference

Catalog No. ABIN125756

Datasheet as PDF

Vimentin antibody

VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunofluorescence staining (rat basophils)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

9 images

4 references

Catalog No. ABIN94491

Datasheet as PDF

SIT1 antibody

SIT1 Reactivity: Human WB, IP, FACS Host: Mouse Monoclonal SIT-01 unconjugated

Western blot of human Jurkat T cell line

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC).

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified antibody (concentration in sample 9 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood).

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions.

Anti-SIT Purified (clone SIT-01) works in WB application.

Anti-SIT Purified (clone SIT-01) specificity verification by WB.

7 images

2 references

Catalog No. ABIN94465

Datasheet as PDF

TUBG1 antibody (C-Term)

TUBG1 Reactivity: Human, Rat, Mouse, Cow, Chicken, Plant, Protozoa, Pig WB, ICC, FACS Host: Mouse Monoclonal TU-30 unconjugated

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions.

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Fig.

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (; direct conjugate with Dyomics 547, red).

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3).

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30, direct conjugate with Dyomics 547, red).

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts.

8 images

8 references

Catalog No. ABIN94313

Datasheet as PDF

Growth Hormone Receptor antibody (AA 101-200)

GHR Reactivity: Human, Mouse, Rat, Pig, Sheep WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Evaluation of the pig GHR transcript with a 4bp insert in vitro.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated (ABIN671481) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human A549 cell lysates probed with Rabbit Anti-GHR Polyclonal Antibody, Unconjugated .

This image was generously provided by Brandon Menzies, PhD, from The University of Melbourne.

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-GHR Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Knockout of the pig GHR gene in F2 homozygous pigs (GHR4bp/4bp refers to -/-).

6 images

7 references

Catalog No. ABIN671481

Datasheet as PDF

HMGB1 antibody (AA 75-170)

HMGB1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (ABIN671616) at 1:400, followed by conjugation to the secondary antibody and DAB staining

Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated .

Formalin-fixed and paraffin embedded rat liver tissue labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

7 references

Catalog No. ABIN671616

Datasheet as PDF

beta Catenin antibody (pSer33, pSer37)

CATNB Reactivity: Human, Mouse, Rat WB, FACS, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phospho-Beta-Catenin(Ser33/37) Polyclonal Antibody, Unconjugated (ABIN683893) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon cancer tissue labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

7 images

1 reference

Catalog No. ABIN683893

Datasheet as PDF

CD28 antibody (AA 1-120)

CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated .

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded Human stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.

7 images

1 reference

Catalog No. ABIN674604

Datasheet as PDF

CCR7 antibody (AA 25-59)

CCR7 Reactivity: Human, Mouse, Rat, Sheep FACS, WB, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, PE-Cy5.

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (ABIN674724) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (ABIN674724) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy5 conjugated used at 1:200 dilution

Formalin-fixed and paraffin embedded human tubular adenoma labeled with Anti-CCR7 CD197 Polyclonal Antibody, unconjugated (ABIN674724) followed by incubation with conjugated secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Raji probed with CCR7/CD197 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

6 images

3 references

Catalog No. ABIN674724

Datasheet as PDF

Cytokeratin 18 antibody (AA 53-150)

KRT18 Reactivity: Human, Mouse, Rat, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (ABIN676973) at 1:200 for 60 minutes at 37C.

Immunofluorescence (IF) image for anti-Keratin 18 (KRT18) (AA 53-150) antibody (ABIN676973)

Formalin-fixed and paraffin embedded rat liver labeled with Anti-CK18 Polyclonal Antibody, Unconjugated (ABIN676973) at 1:800, followed by conjugation to the secondary antibody and DAB staining.

Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Paraformaldehyde-fixed, paraffin embedded human colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.

PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody Alexa Fluor 488 Conjugated Secondary Antibody.

6 images

4 references

Catalog No. ABIN676973

Datasheet as PDF

WNT5A antibody (AA 301-381)

WNT5A Reactivity: Human, Mouse, Rat, Dog WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse ovary lysates probed with WNT5A Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (ABIN675758) at 1:1000 followed by conjugation to the secondary antibody

Image kindly submitted by Dr.

Mouse uterus lysates probed with WNT5A Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (ABIN675758) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image kindly provided by Dr.

6 images

3 references

Catalog No. ABIN675758

Datasheet as PDF

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