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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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77,152 results :

Liver Arginase antibody

KO Validated ARG1 Reactivity: Human WB, ELISA, FACS, IHC (p), IF, IP, ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

WB Image Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE antibody diluted at 1:50000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I , antibody at 1:200 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I , antibody at 1:500 dilution.

WB Image Arginase 1 antibody detects Arginase 1 protein by western blot analysis.

Elevated right ventricular pressure, increased right ventricular weight and altered expression of Ddah1 and Arg1 in cavin-/- mice.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Elevated arginase1 expression and increased arginase inhibitor-induced relaxation of Cavin-1-/- arteries.

Arg-1 expression in neutrophils and lymph-node microenvironmentNeutrophils isolated from control and HL patients were analysed for Arg-1 expression (A), activity by measuring urea (B) and protein expression by Western Blot (C).

8 images

11 references

Catalog No. ABIN2856661

Datasheet as PDF

SQSTM1 antibody

KO Validated SQSTM1 Reactivity: Human WB, IF, IP, IHC (p), FACS, ICC, PLA Host: Rabbit Polyclonal unconjugated

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.

IHC-P Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

WB Image SQSTM1 antibody detects SQSTM1 protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE antibody diluted at 1:1000

WB Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by Western blot analysis.

IP Image Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal, Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

FACS Image SQSTM1 antibody [N3C1], Internal , detects SQSTM1 protein by flow cytometry analysis.

ICC/IF Image SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

Analyses of GMI, TG and TM regulated ER stress, apoptosis and autophagy by Western blotting.

PB induces autophagy but blocks autophagosome trafficking and autophagosome-lysosome fusion in Hep3B cells.

HDAC2 positively regulates the expression of p-Akt, p-mTOR, and survivin in ER+ breast cancer cells.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Effect of autophagy on miR-21-induced drug resistance.

Celastrol activates autophagy and inhibits MPP+-induced neurotoxicity in dopaminergic neurons.

Celastrol enhances the clearance of damaged mitochondria via activating mitophagy.

23 images

13 references

Catalog No. ABIN2854836

Datasheet as PDF

CD25 antibody (AA 201-268)

IL2RA Reactivity: Human, Mouse FACS, WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Immunohistochemistry validation image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody (ABIN670371)

Lane 1: EC9706 lysates Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL2RA Polyclonal Antibody, Unconjugated (ABIN670371) at 1:200 followed by conjugation to the secondary antibody and DAB staining

4 images

Independent Validation

1 validation

Catalog No. ABIN670371

Datasheet as PDF

PALB2 antibody (AA 1101-1186)

PALB2 Reactivity: Human, Pig WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Partner and Localizer of BRCA2 (PALB2) (AA 1101-1186) antibody (ABIN670536)

Images provided by the Independent Validation Program (badge number 29749).

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti PALB2 Polyclonal Antibody, Unconjugated (ABIN670536) at 1:200 followed by conjugation to the secondary antibody and DAB staining

HeLa cells probed with PALB2 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

4 images

Independent Validation

1 validation

Catalog No. ABIN670536

Datasheet as PDF

MEK2 antibody (AA 1-50)

MAP2K2 Reactivity: Human, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500)

Images provided by the Independent Validation Program, badge number 029748.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPKK2 Polyclonal Antibody, Unconjugated (ABIN726500) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250.

4 images

Independent Validation

1 validation

Catalog No. ABIN726500

Datasheet as PDF

FOXO1 antibody (AA 165-270)

FOXO1 Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Chicken WB, ELISA, IF (p), IHC (p), FACS Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody (ABIN1387620)

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (ABIN1387620) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

4 images

Independent Validation

1 validation

Catalog No. ABIN1387620

Datasheet as PDF

BAD antibody (AA 101-204)

BAD Reactivity: Human, Mouse, Rat ELISA, WB, IHC (p), IF (p), IF (cc), FACS, ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-BCL2-Associated Agonist of Cell Death (BAD) (AA 101-204) antibody (ABIN674709)

Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Polyclonal Antibody, Unconjugated (ABIN674709) at 1:300 overnight at 4 °C.

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Bad Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by the Independent Validation Program (badge number 29802).

4 images

Independent Validation

1 validation

Catalog No. ABIN674709

Datasheet as PDF

ALX4 antibody (AA 249-275)

ALX4 Reactivity: Human WB, FACS, CUT&RUN Host: Rabbit Polyclonal RB28072 unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

Cleavage Under Targets and Release Using Nuclease validation image for anti-ALX Homeobox 4 (ALX4) (AA 249-275) antibody (ABIN2844113)

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) western blot analysis in MDA-M cell line lysates (35 μg/lane).

ALX4 Antibody (Center) (ABIN654344 and ABIN2844113) flow cytometric analysis of MDA-M cells (right histogram) compared to a negative control cell (left histogram).

4 images

Independent Validation

1 validation

Catalog No. ABIN654344

Datasheet as PDF

BrdU antibody

BrdU FACS, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal Bu20a unconjugated

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Immunohistochemistry validation image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

Flow Cytometry (FACS) image for anti-Bromodeoxyuridine (BrdU) antibody (ABIN2477713)

3 images

1 reference

Independent Validation

2 validations

Catalog No. ABIN2477713

Datasheet as PDF

pan Keratin antibody

panKRT Reactivity: Mammalian FACS, WB, IHC (p), ICC, IP Host: Mouse Monoclonal C-11 unconjugated

Detection of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 ((ABIN94275), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green).

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/mL, GAM APC).

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

6 images

6 references

Catalog No. ABIN94275

Datasheet as PDF

Integrin beta 2 antibody

ITGB2 Reactivity: Human FACS, WB, IP, IHC (fro), IHC (p) Host: Mouse Monoclonal MEM-48 unconjugated

Fig.

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (Commercially tested by LifeSpan BioSciences.

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 ().

Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/mL, GAM APC).

6 images

5 references

Catalog No. ABIN94005

Datasheet as PDF

ICAM1 antibody

ICAM1 Reactivity: Human, Rat, Cow WB, FACS, ELISA, IHC (p), ICC Host: Mouse Monoclonal MEM-111 unconjugated

Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions.

Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by antibody MEM-111.

Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE.

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD14 (MEM-15) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD54 (MEM-111) purified antibody (concentration in sample 0.

6 images

7 references

Catalog No. ABIN94177

Datasheet as PDF

Transferrin Receptor antibody

TFRC Reactivity: Human FACS, IP, ICC Host: Mouse Monoclonal MEM-75 unconjugated

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE .

Immunofluorescence staining of CD71 in human HeLa cell line using anti-CD71 (; green).

Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (MEM-75, green).

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using anti-human CD3 (UCHT1) PE antibody (20 μL reagent / 100 μL of peripheral whole blood) and anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

Separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) purified antibody (concentration in sample 0.

6 images

1 reference

Catalog No. ABIN94221

Datasheet as PDF

Cytokeratin 18 antibody

KRT18 Reactivity: Human WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal DC-10 unconjugated

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line, negative control) under non-reducing and reducing conditions.

Detectionof cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10 .

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0,6 μg/mL, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 ((ABIN94282), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

6 images

4 references

Catalog No. ABIN94282

Datasheet as PDF

CD247 antibody (Tyr72)

CD247 Reactivity: Human, Mouse WB, FACS, ICC Host: Mouse Monoclonal EM-26 unconjugated

Anti-Hu CD3 zeta (pY72) Purified (clone EM-26) specificity verification by WB.

Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants.

Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed between the lymph node naïve T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Immunocytochemistry detection of phosphorylated CD3 zeta (EM-26, light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min.

Anti-Hu CD3 zeta (p72) purified antibody (clone EM-26) works in Flow Cytometry application Analysis of the antibody staining was performed on Jurkat cells treated or untreated with pervanadate (PV) prior to the fixation and permeabilization of cell suspension with cold methanol.

Separation of pervanadate stimulated Jurkat cell suspension stained using anti-human CD3 zeta (pY72) (EM-26) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from pervanadate stimulated Jurkat cell suspension unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

6 images

1 reference

Catalog No. ABIN2749060

Datasheet as PDF

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