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FACS Antibodies

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It sorts a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell and its attached antibodies.

Important Targets

Arginase 1 Antibodies

CASP8 Antibodies

CCR2 Antibodies

CD14 Antibodies

FOXP3 Antibodies

IL17 Antibodies

Lamin B1 Antibodies

All Flow Cytometry Antibodies

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77,148 results :

Histone 3 antibody (AA 71-136)

H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

K562 Cell lysates probed with Histone H3 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated .

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (ABIN739109) at 1:200 followed by conjugation to the secondary antibody and DAB staining

6 images

13 references

Catalog No. ABIN739109

Datasheet as PDF

alpha Tubulin antibody

TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Eisenia fetida, Turkey, Paramecium WB, IHC (p), IP, ICC, FACS Host: Mouse Monoclonal TU-01 unconjugated

Anti-alpha-Tubulin Purified (TU-01) works in WB application under reducing conditions.

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (; red).

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01 ; green) and anti-Vimentin (VI-01; red).

Nucleus is stained with DAPI (blue).

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Use of anti-alpha-tubulin antibody as a loading control (A) in an Western blotting experiment revealing the staining pattern ofvarious cell lysates by a newly developed monoclonal antibody (B).

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01.

Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01, green) and anti-Vimentin (VI-01, cat.

Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01, red).

Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01, green).

Immunohistochemistry staining of human heart (paraffin sections) using anti-alpha-tubulin (TU-01).

Separation of HeLa cells stained using anti-alpha-Tubulin (TU-01) purified antibody (concentration in sample 3 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Western blotting analysis of human alpha-tubulin using mouse monoclonal antibody TU-01 on lysates of various cell lines under reducing and non-reducing conditions.

13 images

20 references

Catalog No. ABIN93891

Datasheet as PDF

TUBB3 antibody (N-Term)

TUBB3 Reactivity: Human, Mouse, Rat, Dog, Pig WB, FACS, IHC (p), ICC Host: Mouse Monoclonal TU-20 unconjugated

Western blotting analysis of human betaIII-tubulin using mouse monoclonal antibody TU-20 on lysates of U87 cells and Neuro 2a cells (and Jurkat cells as a negative control) under reducing and non-reducing conditions.

Immunohistochemistry staining of betaIII tubulin (red) in tissue sections of murine brain expressing GFP in some of its neurons (green).

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 ; green; 3 μg/ml).

Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunocytochemistry staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20, green, 3 μg/mL).

Immunocytochemistry staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.

Immunohistochemistry staining of human brain (paraffin sections) using anti-betaIII tubulin (TU-20).

Separation of MCF-7 cells stained using anti-betaIII-Tubulin (TU-20) purified antibody (concentration in sample 9 μg/mL, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

9 images

15 references

Catalog No. ABIN93911

Datasheet as PDF

Collagen Type I antibody (AA 1321-1400)

COL1 Reactivity: Human, Rat, Mouse, Rabbit, Dog, Fish WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS Host: Rabbit Polyclonal unconjugated

SW480 lysates probed with Rabbit Anti-Pro-Collagen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:300 followed by conjugation to the secondary PE antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (ABIN670386) 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse muscle lysates probed with Rabbit Anti-Pro-Collegen I Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded dog bladder tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

16 references

Catalog No. ABIN670386

Datasheet as PDF

CD34 antibody (AA 301-385)

CD34 Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Goat FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse heart tissue labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Mouse SP20 Myeloma Cells

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled Anti-CD34 Polyclonal Antibody, Unconjugated (ABIN671361) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, AF488 conjugated used at 1:200 dilution for 40 minutes at 37°C

Mouse brain lysates probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Mouse lung lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Antigen: 0.

6 images

21 references

Catalog No. ABIN671361

Datasheet as PDF

NRF2 antibody (pSer40)

NFE2L2 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody , Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody (ABIN676673), Unconjugated 1:200 followed by conjugation to the secondary Cy5 antibody and DAPIstaining

L1 mouse heart, L2 mouse muscle lysates probed (ABIN676673) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (ABIN676673) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 images

19 references

Catalog No. ABIN676673

Datasheet as PDF

PTGS2 antibody (AA 501-604)

PTGS2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse brain lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated (ABIN672471) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Human A549 lysates probed with Rabbit Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Cyclooxygenase 2/COX2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Effects of Celecoxib on COX-2 and cyclin D1 activation in SKOV-3 xenograft tumors: (A) Western blot analysis of SKOV-3 tumors treated with a high-dose of Celecoxib (100 mg/kg).

COX protein levels in xenograft tumors of nude mice treated or not treated with combined treatment of SC-560 and celecoxib.

COX-2 over expressed in MDR-Caco-2 cells, which could be reversed by sinomenine.

9 images

14 references

Catalog No. ABIN672471

Datasheet as PDF

HIF1A antibody (AA 341-450)

HIF1A Reactivity: Human, Mouse, Rat, Rabbit WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

HL-60 cell lysates probed with Anti-HIF-1 Alpha Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody (ABIN672546), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image kindly submitted by Edita Aksamitiene as part of the Free Sample Program.

HeLa cells probed with HIF-1 Alpha Polyclonal Antibody, unconjugated at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (ABIN672546) at 1:200 in 4 °C.

KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated followed by a FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded human cervical carcinoma labeled Anti-HIF-1-Alpha Polyclonal Antibody , Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

7 images

10 references

Catalog No. ABIN672546

Datasheet as PDF

Beclin 1 antibody (AA 201-330)

BECN1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

293T lyates probed with Rabbit Anti-Beclin 1 Polyclonal Antibody, Unconjugated at 1:300 in 4˚C overnight.

Lane 1: mouse brain lysates Lane 2: mouse intestine lysates probed with Anti BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) at 1:200 in 4C.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat stomach labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugatedused at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rabbit myocardium labeled with Anti-BECN1/Beclin 1/ATG6 Polyclonal Antibody, Unconjugated (ABIN675384) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beclin 1/ATG6 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

8 images

11 references

Catalog No. ABIN675384

Datasheet as PDF

CD90 antibody (AA 31-120)

THY1 Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Sheep fat cells stained withAnti-Thy-1 CD90 Thy1.

Sheep fat cells stained withAnti-Thy-1/CD90/Thy1.

Mouse brain lysates probed with CD90 Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Thy-1/CD90/ Thy1.

Mouse splenocytes probed with Rabbit Anti-Thy-1 Polyclonal Antibody, PE-Cy5.

6 images

23 references

Catalog No. ABIN728038

Datasheet as PDF

VEGF antibody (AA 27-120)

VEGF Reactivity: Human, Mouse, Rat, Pig, Goat WB, ELISA, IHC (p), IHC (fro), IF (p), FACS Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (ABIN727205) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Antigen: 0.

Cells labeled with Anti-VEGF Polyclonal Antibody (ABIN727205).

HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody Alexa Fluor 488 Conjugated.

Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (ABIN727205) at 1:200 in 4 °C.

6 images

14 references

Catalog No. ABIN727205

Datasheet as PDF

GFAP antibody (AA 51-150)

GFAP Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Image provided by One World Lab validation program.

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei.

RSC96 cells probed with GFAP Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation program.

Astrocytes were stained with GFAP polyclonal antibody .

Formalin-fixed and paraffin embedded mouse spinal cord stained with GFAP Polyclonal Antibody at 1:100 dilution.

8 images

20 references

Catalog No. ABIN726200

Datasheet as PDF

IL-1 beta antibody (AA 101-200)

IL1B Reactivity: Human, Mouse, Rat, Cow, Rabbit, Pig WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro), DB Host: Rabbit Polyclonal unconjugated

Raw264.

GSPIONs do not increase expression of pro-inflammatory cytokines in lung parenchyma.

Antigen: 2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG at 1: 5000; TMB staining Read the data in Microplate Reader by 450nm.

Formalin-fixed and paraffin embedded mouse lung labeled with (ABIN728503) Rabbit Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.

Image was kindly submitted by Dr.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

Effects of ALA on cardiac function, inflammation and autophagy in CLP-induced septic rats.

EPD inhibited the IMQ-activated nucleotide-binding oligomerization domain (Nod)-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome signaling pathway.

8 images

16 references

Catalog No. ABIN728503

Datasheet as PDF

TLR4 antibody (AA 751-835)

TLR4 Reactivity: Human, Mouse, Rat, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse macrophages(RAW264.

Mouse splenocytes probed with Rabbit Anti-TLR4 Polyclonal Antibody .

Mouse macrophages(RAW264.

Image submitted by One World Lab validation program.

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat heart labeled with Rabbit Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse heart lysates probed with Anti TLR4/CD284 Polyclonal Antibody, Unconjugated (ABIN736491) at 1:200 overnight at 4 °C.

8 images

13 references

Catalog No. ABIN736491

Datasheet as PDF

ICAM1 antibody (AA 201-300)

ICAM1 Reactivity: Human, Mouse, Rat, Rabbit WB, FACS, ELISA, IHC (p), IHC (fro), ICC, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (ABIN670806), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

EPD inhibited inflammatory cell infiltration in IMQ-induced psoriasis-like mouse mode.

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54 ICAM-1 Polyclonal Antibody, unconjugated (ABIN670806) at 1: 200,followed by incubation with conjugated secondary antibody (Goat Anti-Rabbit IgG, PE conjugated at 1: 200 for 40 minutes at 37°C).

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (ABIN670806) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (ABIN670806), Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

6 images

16 references

Catalog No. ABIN670806

Datasheet as PDF

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