GPD1 antibody

Catalog No. ABIN99896

Product Details anti-GPD1 Antibody

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This GPD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Glycerol-3-Phosphate Dehydrogenase from other sources is unknown.

Purity: Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody has been assayed against 1.0 µg of Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20.000 to 1:100.000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for GPD1

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: Glycerol-3-Phosphate Dehydrogenase (GPD1 Products)

Synonyms: GPDH antibody, Gpd3 antibody, GPD-C antibody, GPDH-C antibody, HTGTI antibody, CG9042 antibody, DROGPDHA antibody, DmG3PDH antibody, Dmel\\CG9042 antibody, G3PDH antibody, G3pdh antibody, GAPDH antibody, GPD antibody, GPDA antibody, GPDH-1 antibody, Gdh antibody, Gpd antibody, alpha-GPD antibody, alpha-GPDH antibody, alpha-GPDH-1 antibody, alpha-Gpdh antibody, alphaGPDH antibody, alphaGpd antibody, alphaGpdh antibody, alphaGpdh-1 antibody, gpdh antibody, gpdh-1 antibody, sn-Gpdh antibody, AI747587 antibody, Gdc-1 antibody, Gdc1 antibody, mKIAA4010 antibody, gpd1 antibody, wu:fc30a07 antibody, zgc:63859 antibody, MGC79676 antibody, GPD1 antibody, LG3P antibody, zgc:112197 antibody, Gpd1 antibody, gpd1h antibody, gpd1l antibody, wu:fc58b05 antibody, zgc:66051 antibody, zgc:85742 antibody, glycerol-3-phosphate dehydrogenase 1 antibody, Glycerol-3-phosphate dehydrogenase [NAD(+)] antibody, glycerol-3-phosphate dehydrogenase antibody, Glycerol 3 phosphate dehydrogenase antibody, glycerol-3-phosphate dehydrogenase 1 (soluble) antibody, glycerol-3-phosphate dehydrogenase 1 L homeolog antibody, glycerol-3-phosphate dehydrogenase 1c antibody, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic antibody, glycerol-3-phosphate dehydrogenase 1a antibody, glycerol-3-phosphate dehydrogenase 1b antibody, Gpd1 antibody, gpdh-1 antibody, GPD1 antibody, Tb11.02.5280 antibody, Gpdh antibody, gpd1.L antibody, gpd1c antibody, gpd1 antibody, LOC101071719 antibody, gpd1a antibody, gpd1b antibody

Background: Glycerol-3-phosphate dehydrogenase (GPDH) is an enzyme that catalyzes the reversible redox conversion of dihydroxyacetone phosphate (aka glycerone phosphate, outdated) to sn-glycerol 3-phosphate. Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. It is also a major contributor of electrons to the electron transport chain in the mitochondria. Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase (alphaGPDH) and glycerolphosphate dehydrogenase (GPDH). However, glycerol-3-phosphate dehydrogenase is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Anti-Glycerol-3-Phosphate Dehydrogenase Antibody is ideal for investigators involved in glucose engery metabolism esearch. Synonyms: EC 1.1.1.8 antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Gene ID: 100339469, 3043365

UniProt: P08507