GST antibody (Biotin)

Catalog No. ABIN99864

Product Details anti-GST Antibody (Biotin)

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: GST Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Characteristics: Synonyms: goat anti-GST antibody biotin conjugation, biotin conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody

Purification: Anti-GST Antibody, Biotin Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-GST Antibody, Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GST antibody (Biotin) (ABIN99864)

Helleboid, Haug, Lamottke, Zhou, Wei, Daix, Cambula, Rigou, Hum, Walczak: "The Identification of Naturally Occurring Neoruscogenin as a Bioavailable, Potent, and High-Affinity Agonist of the Nuclear Receptor RORα (NR1F1)." in: Journal of biomolecular screening, (2013) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: GST (GST Products)

Background: Background: Biotin conjugated anti-GST antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.