Glucose-6-Phosphate Dehydrogenase antibody

Catalog No. ABIN99776

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Leuconostoc mesenteroides

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Glucose-6-Phosphate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-GLUCOSE-6-PHOSPHATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: GLUCOSE-6-PHOSPHATE DEHYDROGENASE Antibody has been assayed against 1.0 µg of Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1.000 to 1:5.000 of the reconstitution concentration is suggested for this product. Researchers should determine optimal titers for other applications.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose-6-Phosphate Dehydrogenase (G6PD Products)

Synonyms: G6PD1 antibody, g6pd antibody, G6pd antibody, G6pdx antibody, g6pdh antibody, g6pd2 antibody, fj78b06 antibody, wu:fj78b06 antibody, si:dkey-90a13.8 antibody, BA3433 antibody, CORTRD1 antibody, G6PDH antibody, GDH antibody, G28A antibody, G6PD antibody, Gpdx antibody, glucose-6-phosphate dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase (G6PD) antibody, glucose-6-P dehydrogenase antibody, glucose-6-phosphate dehydrogenase L homeolog antibody, glucose-6-phosphate 1-dehydrogenase Zwf antibody, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase antibody, glucose-6-phosphate dehydrogenase X-linked antibody, G6PD antibody, g6pd antibody, g6pD antibody, zwf antibody, LOC100120232 antibody, LACBIDRAFT_188936 antibody, G6pd antibody, g6pd.L antibody, CNG03280 antibody, Tb10.70.5200 antibody, H6PD antibody, G6pdx antibody, H6pd antibody

Background: Synonyms: G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Gene ID: 6063509

UniProt: P11411

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones