Glucose Oxidase antibody

Catalog No. ABIN99713

Product Details anti-Glucose Oxidase Antibody

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Sheep

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-GLUCOSE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: GLUCOSE OXIDASE antibody has been assayed against 1.0 µg of Glucose Oxidase [Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2.000 to 1:10.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.500 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Background: Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Gene ID: 2683500

UniProt: P13006