Glucose Oxidase antibody

Catalog No. ABIN99713

Supplier Product No.: 200-6137-0100

Product Details anti-Glucose Oxidase Antibody

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Sheep

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Supplier Product No.: 200-6137-0100

Supplier: Rockland

Purpose: Glucose Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Characteristics: Synonyms: sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Anti-GLUCOSE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Glucose Oxidase antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,500 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Background: Background: Glucose Oxidase enzyme is widely applied for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Furthermore, glucose oxidase-producing molds such as aspergillus and penicillium species are used for the biological production of gluconic acid.

Gene ID: 2683500

UniProt: P13006