Glucose Oxidase antibody

Catalog No. ABIN99709

Product Details anti-Glucose Oxidase Antibody

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein.
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Glucose Oxidase Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:35.000 - 1:160.000
Immunohistochemistry Dilution: 1:2.000 - 1:8.000
Western Blot Dilution: 1:3500 - 1:16.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Synonyms: LOC406081 antibody, GB19418 antibody, glucose oxidase antibody, LOC406081 antibody, AFUA_2G14840 antibody, SS1G_10949 antibody, AOR_1_952194 antibody, AOR_1_786174 antibody, AOR_1_358034 antibody, CC1G_15386 antibody, VDBG_09040 antibody, VDBG_06502 antibody, SCHCODRAFT_113971 antibody

Background: Anti-Glucose Oxidase detects glucose oxidase. Glucose oxidase enzyme (GOx) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids, in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Anti-Glucose Oxidase Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research. Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

UniProt: P13006