Glucose Oxidase antibody
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- Target See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
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Reactivity
- Aspergillus niger
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Glucose Oxidase antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 200-4137-0100
- Supplier
- Rockland
- Purpose
- Glucose Oxidase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
- Characteristics
- Synonyms: rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
- Purification
- Anti-Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Anti-Glucose Oxidase Antibody was produced by repeated immunizations with Aspergillus niger Glucose Oxidase protein.
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Glucose Oxidase (GOD) antibody
GOD Reactivity: Aspergillus niger ELISA, WB, IHC Host: Goat Polyclonal unconjugated
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- Application Notes
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Immunohistochemistry Dilution: 1:2,000 - 1:8,000
Application Note: Anti-Glucose Oxidase Antibody has been tested by ELISA and western blot and is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:3500 - 1:16,000
ELISA Dilution: 1:35,000 - 1:160,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Glucose Oxidase (GOD)
- Alternative Name
- Glucose Oxidase (GOD Products)
- Background
- Background: Anti-Glucose Oxidase detects glucose oxidase. Glucose oxidase enzyme (GOx) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids, in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies. Anti-Glucose Oxidase Antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
- UniProt
- P13006
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