Glucose Oxidase antibody

Catalog No. ABIN99703

Supplier Product No.: 100-1137

Product Details anti-Glucose Oxidase Antibody

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 100-1137

Supplier: Rockland

Purpose: Glucose Oxidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glucose Oxidase [Aspergillus niger].

Characteristics: Synonyms: goat anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Anti-glucose oxidase antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-glucose oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product. Optimal concentration should be determined by the researcher.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Background: Background: Glucose oxidase antibody is specific to glucose oxidase enzyme. Glucose oxidase is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. Glucose oxidase is commonly used for the determination of free glucose in body fluids (diagnostics), and in the food industry. It also has many applications in biotechnologies, for example enzyme assays for biochemistry including biosensors in nanotechnologies. Glucose oxidase is often extracted from Aspergillus niger.

UniProt: P13006