Fructosyl Amino Acid Oxidase antibody
-
- Target See all Fructosyl Amino Acid Oxidase (socD) products
- Fructosyl Amino Acid Oxidase (socD)
-
Reactivity
- Corynebacterium
-
Host
-
Sheep
-
Clonality
- Polyclonal
-
Conjugate
- This Fructosyl Amino Acid Oxidase antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- Supplier Product No.
- 200-601-236-0100
- Supplier
- Rockland
- Purpose
- Fructosyl-Amino Acid Oxidase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Fructosyl-Amino Acid Oxidase [E.
- Characteristics
- Synonyms: sheep anti-Fructosyl-Amino Acid Oxidase Antibody, Fructosyl-amino acid oxidase
- Purification
- Anti-FRUCTOSYL-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
-
Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
Immunogen Type: Recombinant Protein
- Isotype
- IgG
-
-
- Application Notes
-
Application Note: Fructosyl-amino acid oxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Fructosyl-Amino Acid Oxidase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- Fructosyl Amino Acid Oxidase (socD)
- Alternative Name
- Fructosyl-Amino Acid Oxidase (socD Products)
- Background
- Background: Fructosyl amino acid oxidase is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide. Anti-Fructosyl-amino acid oxidase can be useful for researchers interested in diabetes research.
- UniProt
- Q8RIU8
-
