PFKM antibody

Catalog No. ABIN99443

Supplier Product No.: 100-1156

Product Details anti-PFKM Antibody

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PFKM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-1156

Supplier: Rockland

Purpose: Fructose-6-Phosphate Kinase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].

Characteristics: Synonyms: goat anti-Fructose-6-Phosphate Kinase Antibody, 6 Phosphofructokinase Muscle Type antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: This antibody suitable for use in ELISA, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. 

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-PFKM antibody (ABIN99443)

Nakamura, Mori, Eddy: "Molecular complex of three testis-specific isozymes associated with the mouse sperm fibrous sheath: hexokinase 1, phosphofructokinase M, and glutathione S-transferase mu class 5." in: Biology of reproduction, Vol. 82, Issue 3, pp. 504-15, (2010) (PubMed).

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM (PFKM Products)

Background: Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation.  This is a unidirectional and rate-limiting step in glycolysis.  Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate.  The enzyme exists as a homotetramer.

Gene ID: 100345647, 125128

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect