FTH1 antibody

Catalog No. ABIN99122

Supplier Product No.: 200-401-090-0100

Product Details anti-FTH1 Antibody

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 200-401-090-0100

Supplier: Rockland

Purpose: Ferritin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Characteristics: Synonyms: rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Purification: Anti-Ferritin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Ferritin has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:5,000 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-FTH1 antibody (ABIN99122)

Mercer, Ohashi, Boeing, Jefferies, De Tito, Flynn, Tremel, Zhang, Wirth, Frith, Snijders, Williams, Tooze: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).

Mai, Hamaï, Hienzsch, Cañeque, Müller, Wicinski, Cabaud, Leroy, David, Acevedo, Ryo, Ginestier, Birnbaum, Charafe-Jauffret, Codogno, Mehrpour, Rodriguez: "Salinomycin kills cancer stem cells by sequestering iron in lysosomes." in: Nature chemistry, Vol. 9, Issue 10, pp. 1025-1033, (2019) (PubMed).

Goodwin, Dowdle, DeJesus, Wang, Bergman, Kobylarz, Lindeman, Xavier, McAllister, Nyfeler, Hoffman, Murphy: "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9." in: Cell reports, Vol. 20, Issue 10, pp. 2341-2356, (2018) (PubMed).

Kimura, Jia, Kumar, Choi, Gu, Mudd, Dupont, Jiang, Peters, Farzam, Jain, Lidke, Adams, Johansen, Deretic: "Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy." in: The EMBO journal, Vol. 36, Issue 1, pp. 42-60, (2017) (PubMed).

Mancias, Pontano Vaites, Nissim, Biancur, Kim, Wang, Liu, Goessling, Kimmelman, Harper: "Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis." in: eLife, Vol. 4, (2016) (PubMed).

Mancias, Wang, Gygi, Harper, Kimmelman: "Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy." in: Nature, Vol. 509, Issue 7498, pp. 105-9, (2014) (PubMed).

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: FTH1 (FTH1 Products)

Background: Background: Ferritin stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis