Ferritin antibody

Catalog No. ABIN99122

Product Details anti-Ferritin Antibody

Target: Ferritin (FE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ferritin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Ferritin from other tissues and species may occur but have not been specifically determined.

Purity: Anti-FERRITIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Endotoxin Level: Low Endotoxin : No

Immunogen: Ferritin [Human Spleen]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: ELISA Dilution: 1:5.000 - 1:20.000
Immunohistochemistry Dilution: 1:200 - 1:1.000
Western Blot Dilution: 1:5.000 - 1:2.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-Ferritin antibody (ABIN99122)

Mercer, Ohashi, Boeing, Jefferies, De Tito, Flynn, Tremel, Zhang, Wirth, Frith, Snijders, Williams, Tooze: "Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy." in: The EMBO journal, Vol. 40, Issue 14, pp. e105985, (2021) (PubMed).

Mai, Hamaï, Hienzsch, Cañeque, Müller, Wicinski, Cabaud, Leroy, David, Acevedo, Ryo, Ginestier, Birnbaum, Charafe-Jauffret, Codogno, Mehrpour, Rodriguez: "Salinomycin kills cancer stem cells by sequestering iron in lysosomes." in: Nature chemistry, Vol. 9, Issue 10, pp. 1025-1033, (2019) (PubMed).

Goodwin, Dowdle, DeJesus, Wang, Bergman, Kobylarz, Lindeman, Xavier, McAllister, Nyfeler, Hoffman, Murphy: "Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9." in: Cell reports, Vol. 20, Issue 10, pp. 2341-2356, (2018) (PubMed).

Kimura, Jia, Kumar, Choi, Gu, Mudd, Dupont, Jiang, Peters, Farzam, Jain, Lidke, Adams, Johansen, Deretic: "Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy." in: The EMBO journal, Vol. 36, Issue 1, pp. 42-60, (2017) (PubMed).

Mancias, Pontano Vaites, Nissim, Biancur, Kim, Wang, Liu, Goessling, Kimmelman, Harper: "Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis." in: eLife, Vol. 4, (2016) (PubMed).

Mancias, Wang, Gygi, Harper, Kimmelman: "Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy." in: Nature, Vol. 509, Issue 7498, pp. 105-9, (2014) (PubMed).

Target Details for Ferritin

Target: Ferritin (FE)

Alternative Name: Ferritin (FE Products)

Synonyms: 1HCH antibody, BEST:LD36673 antibody, CG2216 antibody, Dmel\\CG2216 antibody, FER1 antibody, FER1HCH antibody, Fer-L antibody, Fer1 antibody, Fer1HC antibody, FerHCH antibody, HCH antibody, T21 antibody, T9 antibody, fer1 antibody, fer1hch antibody, l(3)00451 antibody, l(3)j10B4 antibody, SFerH-1 antibody, SOF-35 antibody, SOF-5L antibody, NV13011 antibody, ACP1 antibody, F8A5.24 antibody, F8A5_24 antibody, Ferritin 1 heavy chain homologue antibody, ferritin light chain antibody, ferritin antibody, chromosome 26 open reading frame, human C10orf131 antibody, hyaluronan and proteoglycan link protein 2 antibody, NAD(P)-linked oxidoreductase superfamily protein antibody, Fer1HCH antibody, H-1 antibody, YPDSF_1340 antibody, ftnA antibody, Ft antibody, C26H10orf131 antibody, HAPLN2 antibody, AT1G60730 antibody

Background: Synonyms: Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis