FTH1 antibody

Catalog No. ABIN99121

Supplier Product No.: 100-401-090

Product Details anti-FTH1 Antibody

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 100-401-090

Supplier: Rockland

Purpose: Ferritin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Ferritin [Human Spleen].

Characteristics: Synonyms: rabbit anti-ferritin antibody, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Ferritin has been tested by immunohistochemistry and is suitable in western blot and to be assayed against 1.0 μg of Ferritin [Human Spleen] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-FTH1 antibody (ABIN99121)

Umehara, Karhanek, Davis, Pourmand: "Label-free biosensing with functionalized nanopipette probes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 12, pp. 4611-6, (2009) (PubMed).

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: FTH1 (FTH1 Products)

Background: Background: Ferritin stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis