FE
Reactivity: Human
ELISA, LF
Host: Chicken
Polyclonal
unconjugated
Application Notes
This product has been assayed against 1.0 ug of Ferritin [Human Spleen] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
90 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Umehara, Karhanek, Davis, Pourmand: "Label-free biosensing with functionalized nanopipette probes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 12, pp. 4611-6, (2009) (PubMed).