TBP antibody (AA 39-50)

Catalog No. ABIN987280

Product Details anti-TBP Antibody

Target: TBP (TATA Box Binding Protein (TBP))

Binding Specificity: AA 39-50

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: TBP /Transcription factor IID (aa39-50)

Sequence: TPQPIQNTNS LS

Specificity: This antibody is expected to recognize both reported isoforms (NP_003185.1, NP_001165556.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-TPQPIQNTNSLS, from the internal region of the protein sequence according to NP_003185.1, NP_001165556.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in nuclear, but not in cytosolic lysates of cell line HeLa (calculated MW of 37.7 kDa according to NP_003185.1) and 37 kDa observed in lysates of NIH3T3 and of Mouse and Rat Testis lysates (calculated MW of 34.7 kDa

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP (TBP Products)

Background: TBP, TATA box binding protein, GTF2D, GTF2D1, HDL4, MGC117320, MGC126054, MGC126055, SCA17, TFIID, TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor, TATA-box-binding protein, transcription initiation factor TFIID

Gene ID: 6908, 21374, 117526

NCBI Accession: NP_003185, NP_001165556

Pathways: WNT Signaling