Elastase antibody
Rockland 100-4168
Reactivity: Pig
ELISA, WB, IHC
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Elastase Antibodies
- Elastase
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Elastase antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 100-4168
- Supplier
- Rockland
- Purpose
- Elastase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Elastase [Porcine Pancreas].
- Characteristics
- Synonyms: rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody
- Purification
- Elastase antibody was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Elastase [Porcine Pancreas]
Immunogen Type: Native Protein
- Top Product
- Discover our top product Elastase Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Elastase antibody is suitable for ELISA and western blot. Usage in other immunoassays such as immunohistochemistry is expected but has not been determined. Optimal concentrations should be determined by researcher.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 80 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Elastase
- Abstract
- Elastase Products
- Background
- Background: Anti elastase antibody detects elastase enzyme. Elastase breaks down elastin, an elastic fiber that, together with collagen, determines the mechanical properties of connective tissue. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine.
- Gene ID
- 396766
- NCBI Accession
- NP_998988
- UniProt
- P00772
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