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Dextranase antibody

Rockland 100-401-089 Reactivity: Penicillium sp. WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN98392
Supplier Product No.: 100-401-089
  • Target See all Dextranase products
    Dextranase
    Reactivity
    Penicillium sp.
    Host
    • 10
    Rabbit
    Clonality
    • 10
    Polyclonal
    Conjugate
    • 4
    • 4
    • 1
    • 1
    This Dextranase antibody is un-conjugated
    Application
    • 10
    • 8
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Supplier Product No.
    100-401-089
    Supplier
    Rockland
    Purpose
    Dextranase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Dextranase [Penicillium species].
    Characteristics
    Synonyms: rabbit anti-Dextranase antibody, Alpha-1-6-glucan-6-glucanohydrolase, DEX
    Purification
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Dextranase [Penicillium species]

    Immunogen Type: Native Protein

  • Application Notes

    Application Note: Anti-Dextranase has been tested by western blot and is suitable to be assayed against 1.0 μg of Dextranase [Penicillium species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethyl-benthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    90 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Dextranase
    Abstract
    Dextranase Products
    Background
    Background: Dextranase has catalytic activity: endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
    UniProt
    P48845
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