DNASE1 antibody

Catalog No. ABIN98343

Product Details anti-DNASE1 Antibody

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Deoxyribonuclease A from other tissues and species may occur but have not been specifically determined.

Purity: Anti-DEOXYRIBONUCLEASE I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas].

Endotoxin Level: Low Endotoxin : No

Immunogen: Deoxyribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-DEOXYRIBONUCLEASE I has been assayed against 1.0 µg of Deoxyribonuclease A [Bovine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1.500 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: Deoxyribonuclease I (DNASE1 Products)

Synonyms: LOC397802 antibody, DNL1 antibody, DRNI antibody, AI788650 antibody, DNaseI antibody, Dnl1 antibody, dnase1-A antibody, zgc:92440 antibody, DNase I antibody, deoxyribonuclease 1 antibody, deoxyribonuclease I antibody, deoxyribonuclease I L homeolog antibody, Deoxyribonuclease I antibody, endA antibody, LOC397802 antibody, DNASE1 antibody, Dnase1 antibody, dnase1.L antibody, dnase1 antibody, CpipJ_CPIJ011184 antibody, CpipJ_CPIJ011185 antibody, CpipJ_CPIJ011186 antibody, CpipJ_CPIJ011187 antibody, CpipJ_CPIJ011190 antibody, CpipJ_CPIJ017803 antibody, Desal_1409 antibody, MPET_RS05255 antibody, METEV_RS02275 antibody, Plabr_0631 antibody

Background: Synonyms: Deoxyribonuclease-1 EC=3.1.21.1 Deoxyribonuclease I DNase I

Gene ID: 282217

UniProt: P00639