Cyclin B1 antibody

Catalog No. ABIN97948

Supplier Product No.: 100-401-152

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 100-401-152

Supplier: Rockland

Purpose: Cyclin B1 Antibody

Cross-Reactivity (Details): Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.

Characteristics: Synonyms: rabbit anti-cyclin B1 antibody, G2/mitotic-specific cyclin-B1

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Cyclin B1 antibody (ABIN97948)

Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, (2014) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1 (CCNB1 Products)

Background: Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Gene ID: 891, 14327896

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase