Cyclin A antibody

Catalog No. ABIN97945

Supplier Product No.: 100-401-151

Product Details anti-Cyclin A Antibody

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 100-401-151

Supplier: Rockland

Purpose: Cyclin A Antibody

Cross-Reactivity (Details): Antiserum will specifically react with a 60 kDa Cyclin A protein from human, rat and mouse tissue.

Characteristics: Synonyms: rabbit anti-Cyclin A antibody, CCN1 antibody, CCNA antibody, CCNA2 antibody, Cyclin A2 antibody

Purification: Anti-Cyclin A was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Cyclin-A Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Cyclin-A antibody has been tested by western blot and immunohistochemistry and is suitable for the detection by immunoblot, ELISA, IHC, and IP of human, rat and mouse Cyclin A.  Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Cyclin A antibody (ABIN97945)

Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: CCNA2 (CCNA2 Products)

Background: Background: Anti-Cyclin A detects Cyclin A. Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Anti-Cyclin A Antibody is ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.

Gene ID: 890

NCBI Accession: NP_001228

UniProt: P20248

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA