Cyanine antibody (HRP)
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- Target See all Cyanine products
- Cyanine
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Cyanine antibody is conjugated to HRP
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Application
- ELISA
- Supplier Product No.
- 600-603-209
- Supplier
- Rockland
- Purpose
- Cyanine Antibody Peroxidase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, anti-Peroxidase and cyanine conjugated IgG.
- Characteristics
- Synonyms: sheep anti-Cyanine Antibody peroxidase Conjugation, HRP conjugated sheep anti-Cyanine Antibody
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
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Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
Immunogen Type: Other
- Isotype
- IgG
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- Application Notes
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Application Note: This product has been assayed against 1.0 μg of Cyanine conjugated Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. This product has been assayed against 1.0 μg of Cyanine conjugated Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
ELISA Dilution: 1:1,500-1:6,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Cyanine
- Abstract
- Cyanine Products
- Target Type
- Chemical
- Background
- Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Peroxidase. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.
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