Cyanine antibody (FITC)

Catalog No. ABIN97921

Supplier Product No.: 600-602-209

Product Details anti-Cyanine Antibody (FITC)

Target: Cyanine

Host: Sheep

Clonality: Polyclonal

Conjugate: This Cyanine antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Supplier Product No.: 600-602-209

Supplier: Rockland

Purpose: Cyanine Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Sheep Serum and cyanine conjugated IgG.

Characteristics: Synonyms: sheep anti-Cyanine Antibody Fluorescein Conjugation, FITC conjugated sheep anti-Cyanine Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Immunogen Type: Other

Isotype: IgG

Labeling Ratio: 4.0

Application Details

Application Notes:

Flow Cytometry Dilution: User Optimized

Application Note: Anti-Cyanine Fluorescein antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

IF Microscopy Dilution: 1:500-1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Cyanine

Target: Cyanine

Abstract: Cyanine Products

Target Type: Chemical

Background: Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. Anti-Cyanine Fluorescein antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.