Cyanine antibody (Biotin)

Catalog No. ABIN97920

Supplier Product No.: 600-606-209

Product Details anti-Cyanine Antibody (Biotin)

Target: Cyanine

Host: Sheep

Clonality: Polyclonal

Conjugate: This Cyanine antibody is conjugated to Biotin

Application: ELISA

Supplier Product No.: 600-606-209

Supplier: Rockland

Purpose: Cyanine Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum and cyanine conjugated IgG.

Characteristics: Synonyms: sheep anti Cyanine Antibody Biotin Conjugated

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Application Note: This product has been assayed against 1.0 μg of Cyanine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:8,000-1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Cyanine

Target: Cyanine

Abstract: Cyanine Products

Target Type: Chemical

Background: Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Biotin. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.