This product was assayed against a lysate of 1 x 10^6 Raji B cells by immunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at a dilution of 1:500 to 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat] Anti-CREB-1 (p43) is suitable for detection by immunoblot of human, mouse and rat CREB-1 (p43). This product was also tested in a gel supershift assay and found to be reactive against CREB-1 (p43) using 0.5 to 1.0 µl per assay.
Restrictions
For Research Use only
Format
Liquid
Concentration
2.56 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
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Wierda, Kuipers, van Eggermond, Benard, van Leeuwen, Carluccio, Geutskens, Jukema, Marquez, Quax, van den Elsen: "Epigenetic control of CCR5 transcript levels in immune cells and modulation by small molecules inhibitors." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1866-77, (2012) (PubMed).
Wang, Sasaki, Zhou, Matsumura, Araki, Mezawa, Takai, Chen, Ogata: "Transcriptional regulation of bone sialoprotein gene by interleukin-11." in: Gene, (2011) (PubMed).
Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).
Target
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
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Background
Anti CREB-1 Antibody recognizes CREB (cAMP response element-binding), a cellular transcription factor. CREB binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the neurotrophin BDNF (Brain-derived neurotrophic factor), tyrosine hydroxylase, and many neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone). CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain. Synonyms: Cyclic AMP-responsive element-binding protein 1 cAMP-responsive element-binding protein 1 CREB-1 CREB1