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Goat anti-Human IgG (Chain gamma), (Heavy Chain) Antibody (FITC)

FACS, IM FITC

Catalog No. ABIN969704

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2383

512

424

207

131

129

94

29

28

28

22

9

6

6

6

6

5

2

2

1

1
Chain gamma, Heavy Chain
Reactivity
723

699

602

477

403

243

212

176

147

138

113

112

107

102

82

59

47

28

28

16

12

10

8

5

5

5

3

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Human
Host
1777

1576

572

241

112

111

33

31

21

19

15

11

11

1
Goat
Conjugate
588

416

416

381

282

276

244

118

108

67

66

64

61

58

52

51

48

44

44

44

41

39

35

32

31

31

30

29

29

29

29

29

29

28

28

28

28

28

28

28

27

27

26

26

25

22

21

19

17

17

16
FITC
Application
1953

1837

1807

1520

934

910

652

478

380

236

236

234

106

102

86

67

53

52

31

28

25

21

18

16

11

9

4

4

4

4

3

2

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunomicroscopy (IM)

Specificity

Based on IEP, this antibody reacts with: heavy (gamma) chains on human IgG

No Cross-Reactivity

Cow (Bovine), Horse (Equine), Mouse (Murine), Rabbit

Characteristics

Fluorophore: Fluorescein-5-isothiocyanate (FITC) Amax = 494 nm, Emax = 518 nm. Fluor Protein Ratio: Moles FITC per Mole Antibody.

Purification

Affinity purified using solid phase Human IgG (H and L)
Small amounts of intact IgG may be present.

Purity

> 90 % based on SDS-PAGE

Immunogen

Purified Human IgG, Fc fragment
Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Mouse anti-Human IgG (Fc Region) antibody (PE)
ELISA, FACS Monoclonal JDC-10 IgG1 PE

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG2b PE

Goat anti-Human IgG (Fc Region) antibody (PE) - Preadsorbed
FACS, FM Polyclonal IgG PE F(ab')2 fragment

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Application Notes

This conjugate is suitable for immunomicroscopy and flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. nm
Excitation/Emission wavelength: 494 nm/514 nm

Restrictions

For Research Use only
Format

Liquid

Concentration

1.50 mg/mL

Buffer

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative

Sodium azide

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Product is photosensitive and should be protected from light.

Storage

4 °C
Target

IgG

Abstract

IgG Products

Target Type

Antibody