NEDD8 antibody

Catalog No. ABIN969546

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Purpose: NEDD8 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Clone: 1A7

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NEDD8 antibody (ABIN969546)

Ohki, Funatsu, Konishi, Chiba: "The mechanism of poly-NEDD8 chain formation in vitro." in: Biochemical and biophysical research communications, Vol. 381, Issue 3, pp. 443-7, (2009) (PubMed).

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8 (NEDD8 Products)

Synonyms: NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody

Background:

Description: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Aliases: NEDD-8

Molecular Weight: 9kDa

Gene ID: 4738

HGNC: 4738

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway