NCK1 antibody

Catalog No. ABIN969545

Product Details anti-NCK1 Antibody

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purpose: NCK1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human NCK1 expressed in E. Coli.

Clone: 5B7

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-NCK1 antibody (ABIN969545)

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, (2010) (PubMed).

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, (2008) (PubMed).

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1 (NCK1 Products)

Synonyms: NCK antibody, NCKalpha antibody, nck-1 antibody, NCK1 antibody, 6330586M15Rik antibody, D230010O13Rik antibody, Nck antibody, Nck-1 antibody, Nck1 antibody, nck antibody, nck1-a antibody, nck1-b antibody, nckalpha antibody, NCK adaptor protein 1 antibody, non-catalytic region of tyrosine kinase adaptor protein 1 antibody, NCK adaptor protein 1b antibody, NCK adaptor protein 1 L homeolog antibody, NCK1 antibody, Nck1 antibody, nck1b antibody, nck1.L antibody

Background:

Description: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Aliases: NCK, nck-1, NCKalpha

Molecular Weight: 47kDa

Gene ID: 4690

HGNC: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals